{"product_id":"cho-host-cell-dna-residue-detection-kit-3g-bht20800128","title":"CHO Host Cell DNA Residue Detection Kit (3G)","description":"\u003cdiv class=\"product-block-list__item product-block-list__item--description\"\u003e\n                    \u003cdiv class=\"card\"\u003e\n\u003cdiv class=\"card__header\"\u003e\n                          \u003ch2 class=\"card__title heading h3\"\u003eDescription\u003c\/h2\u003e\n                        \u003c\/div\u003e\n\n                        \u003cdiv class=\"card__section \"\u003e\n                          \u003cdiv class=\"rte text--pull\"\u003e\n                            \u003cdiv class=\"top_tabc proDetail\" data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\" style=\"text-align: center;\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\" style=\"text-align: left;\"\u003e\u003cspan\u003eCHO\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eHost Cell DNA Residue Detection Kit is used for the quantitative analysis of CHO\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ehost cell DNA residuce in intermediate samples, semi-finished and finished products of various biological products.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\" style=\"text-align: left;\"\u003e\u003cspan\u003eThis kit adopts Taqman fluorescent probe and the polymerase chain reaction (PCR) method, which has fg level minimum detection limit and can specifically and quickly detect the residual CHO\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ecell DNA. The kit needs to be used together with the the Residual DNA Sample Preparation Kit (Cat# 18461ES\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003eor 18469).\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 align=\"justify\" style=\"text-align: left;\"\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eFeatures\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cstrong\u003eHigh anti-interference capability\u003c\/strong\u003e – Reliable performance in complex sample matrices; spike recovery rates consistently between 70–130%.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cstrong\u003eHigh sensitivity \u003c\/strong\u003e– Lower limit of quantification (LLOQ) as low as 0.3 fg\/μL.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cstrong\u003eExcellent precision\u003c\/strong\u003e – Intra-assay CV \u0026lt;10%; inter-assay (intermediate precision) CV \u0026lt;15%.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cstrong\u003eStrong specificity \u003c\/strong\u003e– Exclusively detects CHO residual DNA without interference from other genomic DNA.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cstrong\u003eComprehensive validation \u003c\/strong\u003e– Fully validated according to ICH Q2 (R2) guidelines; complete validation reports available.\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cstrong\u003eRegulatory compliance\u003c\/strong\u003e – Verified in accordance with EP 2.6.7, JP G3, and USP \u0026lt;63\u0026gt; pharmacopeia requirements, meeting international standards.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" style=\"text-align: left;\"\u003e\u003cstrong\u003eQuality assurance\u003c\/strong\u003e – All enzyme raw materials are self-developed and industrialized, ensuring stable supply and consistent quality.\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\" style=\"text-align: left;\"\u003e\n\u003cb\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable class=\"MsoTableGrid\" border=\"1\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"26.5000%\" valign=\"center\"\u003e\n\u003cp\u003eComponents No.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"35.4200%\" valign=\"center\"\u003e\n\u003cp\u003eName\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"18.9800%\" valign=\"center\"\u003e\n\u003cp\u003e41332ES50\u003c\/p\u003e\n\u003cp\u003e(50 T)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"19.0600%\" valign=\"center\"\u003e\n\u003cp\u003e41332ES60\u003c\/p\u003e\n\u003cp\u003e(100 T)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"26.5000%\" valign=\"center\"\u003e\n\u003cp\u003eA\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"35.4200%\" valign=\"center\"\u003e\n\u003cp\u003eCHO qPCR Mix\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"18.9800%\" valign=\"center\"\u003e\n\u003cp\u003e0.75 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"19.0600%\" valign=\"center\"\u003e\n\u003cp\u003e1.5 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"26.5000%\" valign=\"center\"\u003e\n\u003cp\u003eB\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"35.4200%\" valign=\"center\"\u003e\n\u003cp\u003eCHO Primer\u0026amp;Probe Mix\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"18.9800%\" valign=\"center\"\u003e\n\u003cp\u003e250 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"19.0600%\" valign=\"center\"\u003e\n\u003cp\u003e500 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"26.5000%\" valign=\"center\"\u003e\n\u003cp\u003eC\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"35.4200%\" valign=\"center\"\u003e\n\u003cp\u003eDNA Dilution Buffer\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"18.9800%\" valign=\"center\"\u003e\n\u003cp\u003e2×1.8 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"19.0600%\" valign=\"center\"\u003e\n\u003cp\u003e4×1.8 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"26.5000%\" valign=\"center\"\u003e\n\u003cp\u003eD\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"35.4200%\" valign=\"center\"\u003e\n\u003cp\u003eCHO DNA Control(30 ng\/μL)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"18.9800%\" valign=\"center\"\u003e\n\u003cp\u003e25 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"19.0600%\" valign=\"center\"\u003e\n\u003cp\u003e50 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 class=\"MsoNormal\" style=\"text-align: left;\"\u003e\n\u003cstrong\u003eSpecifications\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable class=\"MsoTableGrid\" border=\"1\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"34.8000%\" valign=\"center\"\u003e\n\u003cp\u003eLinearity\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"65.1800%\" valign=\"center\"\u003e\n\u003cp\u003eStandard curve range: 3 fg\/μL – 300 pg\/μL\u003c\/p\u003e\n\u003cp\u003eCorrelation coefficient (R²): ≥ 0.98\u003c\/p\u003e\n\u003cp\u003eSlope: –3.1 to –3.8\u003c\/p\u003e\n\u003cp\u003eAmplification efficiency: 90%–110%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"34.8000%\" valign=\"center\"\u003e\n\u003cp\u003eAccuracy\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"65.1800%\" valign=\"center\"\u003e\n\u003cp\u003eRecovery rate: 50%–150% (industry standard 70%–130%)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"34.8000%\" valign=\"center\"\u003e\n\u003cp\u003ePrecision\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"65.1800%\" valign=\"center\"\u003e\n\u003cp\u003eRepeatability: CV \u0026lt; 15%\u003c\/p\u003e\n\u003cp\u003eIntermediate precision: CV \u0026lt; 15%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"34.8000%\" valign=\"center\"\u003e\n\u003cp\u003eSensitivity\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"65.1800%\" valign=\"center\"\u003e\n\u003cp\u003eLimit of quantification (LOQ): 0.5 fg\/μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"34.8000%\" valign=\"center\"\u003e\n\u003cp\u003eSpecificity\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"65.1800%\" valign=\"center\"\u003e\n\u003cp\u003eNo interference from other genomic DNA sources\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"34.8000%\" valign=\"center\"\u003e\n\u003cp\u003eStability\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"65.1800%\" valign=\"center\"\u003e\n\u003cp\u003eAccelerated stability: 14 days at 37 °C\u003c\/p\u003e\n\u003cp\u003eFreeze–thaw stability: ≥ 10 cycles\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"34.8000%\" valign=\"center\"\u003e\n\u003cp\u003eBlank Control\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"65.1800%\" valign=\"center\"\u003e\n\u003cp\u003eNo-template control (NTC): In 96 repeated qPCR runs, all Ct values ≥ 35 or no amplification observed\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"34.8000%\" valign=\"center\"\u003e\n\u003cp\u003eApplicable instrument models\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"65.1800%\" valign=\"center\"\u003e\n\u003cp\u003eBio-Rad: CFX96 Optic Module.\u003c\/p\u003e\n\u003cp\u003eThermo Scientific: ABI 7500; ABI Quant Studio 5.\u003c\/p\u003e\n\u003cp\u003eSLAN-96S.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"34.8000%\" valign=\"center\"\u003e\n\u003cp\u003eApplication\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"65.1800%\" valign=\"center\"\u003e\n\u003cp\u003eDetecting the residual DNA content of CHO host cells in products such as antibodies, recombinant proteins, and vaccines produced using CHO cells.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 style=\"text-align: left;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp style=\"text-align: left;\"\u003eThis product should be stored at -25~-15℃ for 1 year.\u003c\/p\u003e\n\u003ch3 style=\"text-align: left;\"\u003e\u003cstrong\u003eFigures\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003c!-- [if !supportLists]--\u003e\u003c!--[endif]--\u003e\u003cstrong\u003e1. Good Linearity\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003e\u003cimg alt=\"Figure 1. Standard curve (left) and amplification curve plot (right) for CHO DNA (3G).\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/1_6b41a5ec-5823-47e4-b941-5f0123f17c80_1024x1024.png?v=1758762269\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cb\u003e\u003cspan\u003eFigure 1. Standard curve (left) and amplification curve plot (right) for CHO DNA (3G).\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cspan\u003eThe linear range of the CHO Host Cell Residual DNA Detection Kit (3G) is 3 fg\/μL to 300 pg\/μL, with an R² value of 1.0 and an amplification efficiency of 99.29%. The coefficient of variation (CV) for detection values across concentrations is \u0026lt;15%.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003c!-- [if !supportLists]--\u003e\u003c!--[endif]--\u003e\u003cstrong\u003e2. Sensitivity: Limit of quantification (LOQ) - 0.3 fg\/μL\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/2_02cd1b60-7ff8-40e4-8167-f11224a05689_1024x1024.png?v=1758762269\" alt=\"Figure 2. qPCR detection of CHO DNA at 0.3 fg\/μL.\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cb\u003e\u003cspan\u003eFigure 2. qPCR detection of CHO DNA at 0.3 fg\/μL.\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003cspan\u003eCHO DNA (3G) at the lowest standard curve concentration (3 fg\/μL) and below was tested with 10 replicates per concentration. Results showed that at concentrations ≥0.3 fg\/μL, the CV was \u0026lt;20%, indicating that the limit of quantification (LLOQ) of the CHO Host Cell DNA Residual Detection Kit (3G) is 0.3 fg\/μL.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e3. \u003c!--[endif]--\u003eHigh Recovery rate: 50%–150%\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eTable 1. Recovery of CHO DNA in Blank Samples\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/3_f1789d7e-0b5e-4008-8e1b-8cfcad532d70_1024x1024.png?v=1758762268\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003cspan\u003eSpike recovery rates for all concentrations tested were between 70% and 130%, with coefficients of variation (CV) below 20%, demonstrating consistent performance of the assay across a wide dynamic range.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cstrong\u003eTable 2. CHO DNA Recovery in Various Sample Matrices\u003c\/strong\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cspan\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/4_cfac5acf-b60a-4e96-b344-1e13c65b5dbb_1024x1024.png?v=1758762268\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003cspan\u003eCHO DNA (300 fg\/μL) was spiked into five different background matrices (high protein, high nucleic acid, high salt, high pH, low pH) and extracted using the same procedure. Recovery rates ranged from 70% to 130%, with CVs below 20% for all matrices, demonstrating robust performance across diverse sample backgrounds.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"p\" style=\"text-align: left;\"\u003e\n\u003cb\u003e\u003cspan\u003eDocuments\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\" style=\"text-align: left;\"\u003e\u003cspan\u003eSafety Data Sheet\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/41332-MSDS-HB250924.pdf?v=1758761916\"\u003e41332_MSDS_HB250924_EN.PDF\u003c\/a\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003eManuals\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"top_tabc\" data-v-444b0c7e=\"\" style=\"text-align: left;\"\u003e\n\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/41332ES-CHO_Host_Cell_DNA_Residue_Detection_Kit_3G_-Ver.EN20240517.pdf?v=1733385399\"\u003e\u003cspan id=\"tabCBox1\" class=\"boxTap\" data-v-444b0c7e=\"\"\u003e41332_Manual_Ver.EN20240517.PDF\u003c\/span\u003e\u003c\/a\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv class=\"cwrap\" data-v-98c835fc=\"\" data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv class=\"list\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv class=\"title\" data-v-98c835fc=\"\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\n                          \u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n                  \u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"50 T","offer_id":53339939078509,"sku":"41332ES50","price":1555.0,"currency_code":"USD","in_stock":true},{"title":"100 T","offer_id":53339941306733,"sku":"41332ES60","price":2805.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1_1024x1024_789d4a81-9a9c-42b5-ac70-da5de46b9c37.png?v=1779018080","url":"https:\/\/www.ebiohippo.com\/products\/cho-host-cell-dna-residue-detection-kit-3g-bht20800128","provider":"BioHippo","version":"1.0","type":"link"}