{"product_id":"chp2-antibody-calcineurin-b-homologous-protein-2-bha17135617","title":"CHP2 Antibody \/ Calcineurin B homologous protein 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eCHP2 Antibody \/ Calcineurin B homologous protein 2 is a anti-CHP2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Cytoplasm, nucleus, cell membrane.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e CHP2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eCHP2 antibody detects Calcineurin B homologous protein 2 (also known as CHP2 or Calcium-binding protein P22), a calcium-binding regulatory protein that modulates sodium-hydrogen exchanger (NHE) activity and signal transduction. Encoded by the CHP2 gene on chromosome Xq26.1, this EF-hand protein shares strong sequence similarity with CHP1 and serves as a cofactor for NHE family members and calcineurin-like enzymes. CHP2 contains three EF-hand calcium-binding motifs that undergo conformational changes upon Ca2+ binding, allowing dynamic interactions with target proteins and membranes. Through its calcium-sensitive regulatory role, CHP2 influences intracellular pH control, cell proliferation, and migration.\u003cbr\u003e\u003cbr\u003eCHP2 is expressed predominantly in the heart, skeletal muscle, and certain cancers, including colon and pancreatic tumors. It binds directly to the cytoplasmic tail of sodium-hydrogen exchangers (NHE1, NHE2, NHE3) to stabilize and activate their function. Unlike CHP1, which is ubiquitously expressed, CHP2 expression is more tissue-specific and often upregulated in malignant cells. By regulating NHE activity, CHP2 contributes to pH homeostasis essential for tumor cell survival and motility. Overexpression of CHP2 has been linked to enhanced proliferation and metastatic potential through activation of MAPK and AKT signaling pathways.\u003cbr\u003e\u003cbr\u003eThe CHP2 antibody is widely used in cell signaling and cancer metabolism research to study calcium-regulated ion transport and tumor biology. Western blotting typically detects a 22 kilodalton band corresponding to the full-length protein, while immunofluorescence shows cytoplasmic and membrane localization consistent with NHE association. Researchers employ the CHP2 antibody to evaluate calcium-dependent signaling events and to compare CHP isoform expression in normal versus transformed cells. Its use extends to studies on cardiac physiology, as CHP2 modulates Ca2+-sensitive processes and contributes to excitation-contraction coupling.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=CHP2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CHP2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=CHP2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/CHP2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=CHP2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047297311085,"sku":"FY12714","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_db32fb9b-029c-4d6a-9326-77cb23062d52.jpg?v=1782237040","url":"https:\/\/www.ebiohippo.com\/products\/chp2-antibody-calcineurin-b-homologous-protein-2-bha17135617","provider":"BioHippo","version":"1.0","type":"link"}