{"product_id":"ciao3-antibody-cytosolic-iron-sulfur-assembly-component-3-bha17135389","title":"CIAO3 Antibody \/ Cytosolic iron-sulfur assembly component 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eCIAO3 Antibody \/ Cytosolic iron-sulfur assembly component 3 is a anti-CIAO3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e CIAO3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eCIAO3 antibody recognizes Cytosolic iron-sulfur assembly component 3, a core factor involved in the maturation of cytosolic and nuclear iron-sulfur (Fe-S) proteins. CIAO3 functions as part of the cytosolic iron-sulfur protein assembly (CIA) complex, which transfers Fe-S clusters from scaffold proteins to target apoproteins essential for DNA replication, RNA processing, and metabolic reactions. The CIAO3 antibody is commonly used to investigate cellular iron homeostasis, redox biology, and cofactor assembly pathways crucial for enzymatic function.\u003cbr\u003e\u003cbr\u003eCIAO3 is encoded by the CIAO3 gene located on human chromosome 14q32.31. The protein is approximately 28 kilodaltons in size and belongs to the NUBP (nucleotide-binding protein) family of P-loop NTPases. It interacts with other CIA machinery components, including MMS19, CIAO1, and FAM96B (CIAO2B), forming a functional complex responsible for Fe-S cluster transfer to client proteins. CIAO3 contains conserved Walker A and Walker B motifs, enabling nucleotide binding and hydrolysis that regulate its conformational dynamics during cofactor delivery.\u003cbr\u003e\u003cbr\u003eThe CIAO3 antibody detects a 53 kilodalton band by western blot.rafficking. Functional studies show that depletion of CIAO3 results in defective maturation of cytosolic aconitase, DNA polymerases, and helicases, leading to impaired DNA replication and increased oxidative stress sensitivity. CIAO3-deficient cells exhibit reduced activity of Fe-S-dependent enzymes and compromised genome integrity.\u003cbr\u003e\u003cbr\u003eCIAO3 also participates in iron homeostasis and redox regulation by influencing intracellular iron distribution. Disruption of the CIA machinery contributes to mitochondrial dysfunction, neurodegeneration, and metabolic disorders. Given its conserved function across species, CIAO3 serves as a model for understanding Fe-S protein biogenesis in eukaryotes.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=CIAO3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CIAO3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=CIAO3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/CIAO3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=CIAO3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047290397037,"sku":"FY12486","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_2ee8e2c4-0325-4dc2-9943-fffd4f79fd7c.jpg?v=1782237016","url":"https:\/\/www.ebiohippo.com\/products\/ciao3-antibody-cytosolic-iron-sulfur-assembly-component-3-bha17135389","provider":"BioHippo","version":"1.0","type":"link"}