{"product_id":"clpp-antibody-hrp-conjugated-bha10532511","title":"CLPP Antibody, HRP conjugated","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis polyclonal anti-CLPP antibody raised in Rabbit, conjugated with HRP, tested in ELISA. Designed to detect CLPP in Human for signal transduction research.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunogen:\u003c\/strong\u003e Recombinant Human ATP-dependent Clp protease proteolytic subunit, mitochondrial protein (223-277AA) — determines epitope; confirm sample prep compatibility.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost (Rabbit):\u003c\/strong\u003e Requires anti-rabbit-IgG secondary matching isotype IgG.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePolyclonal:\u003c\/strong\u003e Multiple epitopes = robust signal; lot-to-lot variability — include controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype (IgG):\u003c\/strong\u003e Match secondary, Protein A\/G, and isotype controls to IgG.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification (Protein G purification):\u003c\/strong\u003e Enriches Ig classes; reduces non-specific background.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHRP:\u003c\/strong\u003e Direct detection (TMB\/ECL); quench endogenous peroxidase in tissue assays.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eCLPP\u003c\/strong\u003e (also: ATP dependent protease ClpAP (E coli) proteolytic subunit, ATP dependent protease ClpAP proteolytic subunit, ATP dependent protease ClpAP proteolytic subunit human, ATP dependent proteolytic subunit homolog (E coli), ATP dependent proteolytic subunit homolog, Caseinolytic peptidase ATP dependent proteolytic subunit homolog, Caseinolytic protease ATP dependent proteolytic subunit E coli, clpP, ClpP caseinolytic peptidase ATP dependent proteolytic subunit, ClpP caseinolytic peptidase ATP dependent proteolytic subunit homolog, CLPP_HUMAN, Endopeptidase Clp, mitochondrial, Putative ATP dependent Clp protease proteolytic subunit mitochondrial, Putative ATP dependent Clp protease proteolytic subunit, Putative ATP-dependent Clp protease proteolytic subunit) — studied in Human systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current CLPP annotation relevant to signal transduction.\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e Quantify soluble target; ensure samples within the linear detection range.\u003c\/li\u003e\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype controls:\u003c\/strong\u003e IgG from Rabbit at equivalent concentration.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross-reactivity:\u003c\/strong\u003e Polyclonal: validate with KO\/KD controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMatrix effects:\u003c\/strong\u003e Verify dilution linearity in your sample matrix.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReactivity:\u003c\/strong\u003e Confirmed for Human; extrapolation requires validation.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cem\u003eThis product is a polyclonal antibody purified from rabbit antiserum.\u003c\/em\u003e\u003c\/p\u003e\u003c!-- Sources: UniProt, NCBI Gene, Cusabio, Human Protein Atlas, PubMed --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"50 ug","offer_id":53241140347245,"sku":"CSB-PA618094LB01HU-50UG","price":166.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53241230950765,"sku":"CSB-PA618094LB01HU-100UG","price":299.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/clpp-antibody-hrp-conjugated-bha10532511","provider":"BioHippo","version":"1.0","type":"link"}