{"product_id":"cntrl-antibody-centriolin-cep110-bha17135609","title":"CNTRL Antibody \/ Centriolin \/ CEP110","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eCNTRL Antibody \/ Centriolin \/ CEP110 is a anti-CNTRL Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e CNTRL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eCNTRL antibody detects Centriolin (also known as CEP110 or Centriole-associated protein CNTRL), a large coiled-coil protein that localizes to centrosomes and the midbody, where it regulates cytokinesis and cell cycle progression. Encoded by the CNTRL gene on chromosome 9q33.2, this protein functions as a structural scaffold coordinating membrane trafficking, microtubule organization, and abscission during late mitosis. CNTRL interacts with both centrosomal and Golgi-derived vesicle components, linking cytokinetic machinery with secretory pathways required for cell division completion.\u003cbr\u003e\u003cbr\u003eCentriolin contains multiple coiled-coil domains and leucine zipper motifs that mediate self-association and recruitment of other midbody proteins, including exocyst complex components (EXOC6 and EXOC8) and SNARE regulators. During mitosis, CNTRL localizes to the midbody ring structure where it anchors vesicles essential for the final abscission step separating daughter cells. Loss of CNTRL disrupts cytokinesis, leading to multinucleation and cell cycle arrest. The protein also contributes to centrosome cohesion and Golgi organization during interphase, maintaining proper microtubule anchoring and intracellular transport.\u003cbr\u003e\u003cbr\u003eThe CNTRL antibody is widely used in cell biology and cancer research to study centrosome dynamics, cytokinesis, and vesicle trafficking. In immunofluorescence microscopy, CNTRL staining reveals punctate centrosomal and midbody localization, while western blot analysis identifies a high molecular weight band of approximately 230 kilodaltons. Because defects in CNTRL function are associated with abnormal cell division and chromosomal instability, this antibody is valuable for examining mitotic control and tumorigenesis. CNTRL dysregulation has been reported in several cancers, including glioblastoma and ovarian carcinoma, where centrosome amplification and cytokinetic failure contribute to genomic instability.\u003cbr\u003e\u003cbr\u003eBeyond its role in mitosis, CNTRL participates in cilia formation and signaling. It anchors specific vesicles involved in ciliogenesis, linking basal bodies to the Golgi apparatus. The CNTRL antibody enables detection of this dual localization pattern and supports mechanistic studies into centrosome-related diseases (centrosomopathies).\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=CNTRL - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CNTRL - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=CNTRL - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/CNTRL - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=CNTRL+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047296885101,"sku":"FY12706","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_08b87cdb-0818-4cc3-ba9f-788b4b5b5bda.jpg?v=1782237029","url":"https:\/\/www.ebiohippo.com\/products\/cntrl-antibody-centriolin-cep110-bha17135609","provider":"BioHippo","version":"1.0","type":"link"}