{"product_id":"coenzyme-a-free-acid-bhb21200001","title":"Coenzyme A, Free Acid","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eCoenzyme A, Free Acid is a biochemical supplied by Coenza for use in enzymology and metabolic research. Available as Lyophilized powder, purity ≥ 95%, suitable for in vitro assays and pathway studies.\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eAlso known as:\u003c\/em\u003e Coenzyme A, CoA, CoA-SH, Aluzime, Coalip, Thiol-CoA.\u003c\/p\u003e\n\u003ch2\u003eKey Elements and Design Rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormula \/ MW \/ Purity:\u003c\/strong\u003e C21H36N7O16P3S; 767.53 g\/mol; ≥ 95%. The Free Acid form provides enhanced aqueous stability.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eForm \/ Solubility:\u003c\/strong\u003e Lyophilized powder; Soluble in water.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSynonyms:\u003c\/strong\u003e Coenzyme A, CoA, CoA-SH, Aluzime, Coalip, Thiol-CoA.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eOrigin:\u003c\/strong\u003e Biosynthetic synthesis.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eBiological Background\u003c\/h2\u003e\n\u003cp\u003eCoenzyme A (CoA) is a universal metabolic cofactor synthesized from cysteine, pantothenate, and ATP. It is notable for its role in the synthesis and oxidation of fatty acids and the oxidation of pyruvate in the citric acid cycle (Leonardi et al., 2005). All genomes sequenced to date encode enzymes that use coenzyme A as a substrate, and around 4% of cellular enzymes use it, or a thioester form of it, as a substrate (Sibon \u0026amp; Strauss, 2016).ApplicationsCoenzyme A (CoA) Free Acid is widely used in biochemical research and drug development due to its role as a cofactor in numerous enzymatic reactions, particularly in acyl group transfer processes. It is extensively applied in studies of metabolic pathways, such as the citric acid cycle and fatty acid metabolism, to explore enzyme functions, regulatory mechanisms, and potential therapeutic targets (Leonardi et al., 2005). CoA Free Acid is also valuable in drug discovery for evaluating inhibitors or activators of CoA-dependent enzymes, which are crucial for developing treatments for metabolic disorders and infections (Spry et al., 2008).\u003c\/p\u003e\n\u003ch2\u003eResearch Relevance and Current Trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAcyl-CoA metabolism increasingly linked to histone acylation marks and epigenetic regulation in cancer and metabolic disease research.\u003c\/li\u003e\n\u003cli\u003eGrowing interest in short-chain fatty acid CoA thioesters as mediators of gut microbiome–host metabolic crosstalk.\u003c\/li\u003e\n\u003cli\u003eCoA-dependent enzymes investigated as drug targets in infectious disease and neurometabolic disorder research.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon Research Applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEnzyme kinetics assays — direct substrate for acyltransferases, thiolases, and dehydrogenases.\u003c\/li\u003e\n\u003cli\u003eMetabolic flux analysis — isotope-labeled variants available for stable-isotope tracing.\u003c\/li\u003e\n\u003cli\u003eIn vitro pathway reconstitution for fatty acid β-oxidation, TCA cycle, or polyketide biosynthesis.\u003c\/li\u003e\n\u003cli\u003eBiochemical characterization of CoA-binding proteins by activity assays or binding measurements.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eNotes for Experimental Interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCoA thioesters hydrolyze at neutral–alkaline pH; prepare working solutions fresh and keep on ice.\u003c\/li\u003e\n\u003cli\u003eDifferent salt forms share the same core structure — normalize concentrations using the free-acid MW when comparing across forms.\u003c\/li\u003e\n\u003cli\u003eThiol oxidation may occur upon air exposure; use under inert atmosphere or with reducing agents where appropriate.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Coenza","offers":[{"title":"10 mg","offer_id":53249725497709,"sku":"A-02-10MG","price":51.2,"currency_code":"USD","in_stock":true},{"title":"25 mg","offer_id":53249731166573,"sku":"A-02-25MG","price":81.3,"currency_code":"USD","in_stock":true},{"title":"50 mg","offer_id":53249731199341,"sku":"A-02-50MG","price":113.7,"currency_code":"USD","in_stock":true},{"title":"100 mg","offer_id":53249731232109,"sku":"A-02-100MG","price":162.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/c82f39_2461aa08ef184ed0ba8b5afb2bd570d8_mv2.png?v=1776855582","url":"https:\/\/www.ebiohippo.com\/products\/coenzyme-a-free-acid-bhb21200001","provider":"BioHippo","version":"1.0","type":"link"}