{"product_id":"col2a1-antibody-collagen-alpha-1-ii-chain-bha17136157","title":"COL2A1 Antibody \/ Collagen alpha-1(II) chain","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eCOL2A1 Antibody \/ Collagen alpha-1(II) chain is a anti-COL2A1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e COL2A1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eCOL2A1 antibody detects Collagen alpha-1(II) chain, a structural protein that forms the major component of type II collagen found in cartilage, vitreous humor, and intervertebral discs. The UniProt recommended name is Collagen alpha-1(II) chain (COL2A1). This fibrillar collagen provides tensile strength and elasticity to cartilaginous tissues, enabling load-bearing and mechanical resilience in joints and skeletal structures.\u003cbr\u003e\u003cbr\u003eFunctionally, COL2A1 antibody identifies a 1,484-amino-acid extracellular matrix protein synthesized as a procollagen precursor containing N- and C-terminal propeptides. Following secretion, these propeptides are cleaved to form mature triple-helical type II collagen fibrils composed of three identical alpha-1(II) chains. COL2A1 plays a critical role in chondrocyte differentiation, cartilage development, and matrix maintenance. It also interacts with proteoglycans such as aggrecan to form a hydrated matrix that supports compressive resistance.\u003cbr\u003e\u003cbr\u003eThe COL2A1 gene is located on chromosome 12q13.11 and is highly expressed in cartilage, eye, and intervertebral disc tissues. Expression is regulated by transcription factors SOX9, RUNX2, and NFATC1, ensuring precise control during skeletal development and repair. COL2A1 is a key marker for chondrogenic lineage commitment in mesenchymal stem cells and serves as a diagnostic biomarker for cartilage integrity.\u003cbr\u003e\u003cbr\u003ePathologically, mutations in COL2A1 cause a spectrum of disorders collectively known as type II collagenopathies, including Stickler syndrome, spondyloepiphyseal dysplasia congenita, and achondrogenesis type II. These conditions are characterized by skeletal malformations, joint defects, and ocular abnormalities. Degradation or reduced expression of COL2A1 contributes to osteoarthritis and intervertebral disc degeneration. Research using COL2A1 antibody supports studies in cartilage biology, skeletal development, and connective tissue disease.\u003cbr\u003e\u003cbr\u003eCOL2A1 antibody is validated for western blotting, immunohistochemistry, and ELISA to detect extracellular matrix proteins. \u003cbr\u003e\u003cbr\u003eStructurally, Collagen alpha-1(II) chain contains a repeating Gly-X-Y motif that forms the characteristic triple-helical structure of fibrillar collagens. The C-terminal propeptide directs trimer assembly, while extensive post-translational modifications including hydroxylation and glycosylation stabilize fibril formation. This antibody facilitates investigation of COL2A1's role in cartilage architecture, skeletal morphogenesis, and connective tissue health.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=COL2A1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=COL2A1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=COL2A1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/COL2A1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=COL2A1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047320281453,"sku":"FY13255","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_e2243a45-04ee-466e-8683-12de5b8db29d.jpg?v=1782237083","url":"https:\/\/www.ebiohippo.com\/products\/col2a1-antibody-collagen-alpha-1-ii-chain-bha17136157","provider":"BioHippo","version":"1.0","type":"link"}