{"product_id":"colgalt1-antibody-collagen-beta-1-o-galactosyltransferase-1-bha17135559","title":"COLGALT1 Antibody \/ Collagen beta(1-O)galactosyltransferase 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eCOLGALT1 Antibody \/ Collagen beta(1-O)galactosyltransferase 1 is a anti-COLGALT1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e COLGALT1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eCOLGALT1 antibody detects Collagen beta(1-O)galactosyltransferase 1, an enzyme that catalyzes the transfer of galactose to hydroxylysine residues in collagen. This post-translational modification is essential for proper collagen folding, stability, and secretion. COLGALT1 functions within the endoplasmic reticulum and Golgi apparatus and contributes to the biosynthesis of structurally mature collagen fibers. The COLGALT1 antibody is widely used in connective tissue, developmental, and fibrosis research to study collagen maturation, glycosylation, and extracellular matrix regulation.\u003cbr\u003e\u003cbr\u003eCOLGALT1 is encoded by the COLGALT1 gene located on human chromosome 19p13.2. The protein is approximately 739 amino acids long and is a member of the galactosyltransferase family. It forms a functional complex with its homolog COLGALT2 and works sequentially with lysyl hydroxylase (PLOD family) enzymes to glycosylate hydroxylysine residues in collagen alpha chains. This process stabilizes triple helix formation and enhances collagen secretion and cross-linking.\u003cbr\u003e\u003cbr\u003eThe COLGALT1 antibody detects an 85 kilodalton protein by western blot and shows perinuclear staining under immunofluorescence microscopy consistent with Golgi localization. COLGALT1 activity is required for the synthesis of type I, II, III, and IV collagens. Deficiency or mutation results in defective collagen glycosylation, leading to abnormal extracellular matrix assembly and diseases such as Ehlers-Danlos syndrome and osteogenesis imperfecta-like disorders.\u003cbr\u003e\u003cbr\u003eCOLGALT1 expression is tightly regulated during development and tissue repair. Increased expression promotes collagen deposition in fibrotic tissues, whereas reduced expression leads to unstable collagen and tissue fragility. It also contributes to tumor stroma remodeling by enhancing extracellular matrix density and rigidity, influencing cancer cell migration and angiogenesis.\u003cbr\u003e\u003cbr\u003eAs a key enzyme linking collagen biosynthesis and tissue integrity, COLGALT1 serves as a critical target for studying connective tissue disorders and fibrotic diseases.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=COLGALT1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=COLGALT1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=COLGALT1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/COLGALT1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=COLGALT1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047295902061,"sku":"FY12656","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_6aa6654d-0576-49c8-a8b6-096c5468ce42.jpg?v=1782237057","url":"https:\/\/www.ebiohippo.com\/products\/colgalt1-antibody-collagen-beta-1-o-galactosyltransferase-1-bha17135559","provider":"BioHippo","version":"1.0","type":"link"}