{"product_id":"complement-factor-h-antibody-cfh-bha17102816","title":"Complement factor H Antibody \/ CFH","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eComplement factor H antibody supplied as a purified reagent for IHC-P, WB, IF in Human samples. This product is a polyclonal (rabbit origin) antibody (host: Rabbit; isotype: Rabbit IgG) intended for research use only.\u003c\/p\u003e \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003e\n\u003cstrong\u003eAntibody identity:\u003c\/strong\u003e Polyclonal (rabbit origin); host Rabbit; isotype Rabbit IgG.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eFormat and purification:\u003c\/strong\u003e format: Purified; purity: Antigen affinity purified.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eSpecies reactivity (reported):\u003c\/strong\u003e Human.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eApplications (listed):\u003c\/strong\u003e IHC-P, WB, IF.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eImmunogen \/ epitope context:\u003c\/strong\u003e A portion of amino acids 751-780 from the human protein was used as the immunogen for the Complement factor H antibody..\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eThese attributes help you align the antibody with the biological question (target state, sample type, and readout) while keeping interpretation grounded in appropriate controls.\u003c\/p\u003e \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eComplement factor H is the intended antigen for this primary antibody. Reported biological context includes: This gene is a member of the Regulator of Complement Activation (RCA) gene cluster and encodes a protein with twenty short concensus repeat (SCR) domains. This protein is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eSpatial and single-cell approaches: imaging-based and cytometry workflows increasingly quantify heterogeneity and relocalization rather than only bulk abundance.\u003c\/li\u003e   \u003cli\u003eInteraction-centric biology: IP-based enrichment and proteomics are widely used to define complexes, binding partners, and context-specific interactomes.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eIHC-P: commonly used to measure relative target levels or localization changes in the context of the experimental question.\u003c\/li\u003e   \u003cli\u003eWestern blot (WB): compare relative abundance\/isoform patterns across conditions and sample types; band shifts may reflect processing or post-translational modification.\u003c\/li\u003e   \u003cli\u003eImmunofluorescence (IF): visualize localization and co-localization patterns in cells or tissues.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eAcross these readouts, differences in signal intensity, localization, or complex enrichment are typically interpreted alongside sample-matched controls and independent evidence to distinguish regulation from technical variation.\u003c\/p\u003e \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eIsoforms, cleavage products, or post-translational modifications can alter apparent molecular weight and subcellular distribution; interpret bands and staining patterns in the context of expected biology and sample preparation.\u003c\/li\u003e   \u003cli\u003eSpecies differences and epitope conservation may affect binding; use matched positive controls and orthogonal evidence when comparing across organisms.\u003c\/li\u003e   \u003cli\u003eControl concepts: include appropriate isotype and secondary-only controls (for imaging), and consider genetic perturbations (knockout\/knockdown\/overexpression) or independent antibodies targeting distinct epitopes to strengthen conclusions.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eEpitope context is defined by the immunogen description; when available, align this with known domains, PTM sites, or family homology to anticipate potential cross-reactivity patterns. As a polyclonal antibody, recognition spans multiple epitopes, which can improve detection across conformations but may broaden background depending on sample context.\u003c\/p\u003e \u003c!-- Sources (internal): - UniProtKB entry (P08603) — UniProt Consortium — https:\/\/www.uniprot.org\/uniprotkb\/P08603\/entry - NCBI Gene search (Complement factor H) — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=Complement+factor+H - Ensembl search (Complement factor H) — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=Complement+factor+H - PubMed search (Complement factor H) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Complement+factor+H - Reactome pathway search (Complement factor H) — Reactome — https:\/\/reactome.org\/content\/query?q=Complement+factor+H --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"In 1X PBS, pH 7.4, with 0.09% sodium azide \/ 0.08 ml","offer_id":53043217695085,"sku":"F54343-0.08ML","price":205.0,"currency_code":"USD","in_stock":true},{"title":"In 1X PBS, pH 7.4, with 0.09% sodium azide \/ 0.4 ml","offer_id":53043618283885,"sku":"F54343-0.4ML","price":439.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_8784917b-6af7-4db8-9e8f-3482fb811c98.jpg?v=1771934154","url":"https:\/\/www.ebiohippo.com\/products\/complement-factor-h-antibody-cfh-bha17102816","provider":"BioHippo","version":"1.0","type":"link"}