{"product_id":"concanavalin-a-coated-magnetic-beads-bhd20800001","title":"Concanavalin A-Coated Magnetic Beads","description":"\u003cdiv class=\"card\"\u003e\n\u003cdiv class=\"card__header\"\u003e\n                          \u003ch2 class=\"card__title heading h3\"\u003eDescription\u003c\/h2\u003e\n                        \u003c\/div\u003e\n\n                        \u003cdiv class=\"card__section \"\u003e\n                          \u003cdiv class=\"rte text--pull\"\u003e\n                            \u003cp style=\"font-weight: 400;\"\u003eConcanavalin A (ConA) beads are superparamagnetic polymer microspheres that have been covalently coupled with Concanavalin A\u003cspan\u003e \u003c\/span\u003e(a plant mannose\/glucose-binding lectin isolated from the seeds of cereal plants)on their surface. These beads\u003cspan\u003e \u003c\/span\u003eposessess several notable characteristics, including monodispersity and strong magnetic reactivity. When Ca\u003csup\u003e2+\u003c\/sup\u003e and Mg\u003csup\u003e2+\u003c\/sup\u003e ions are present, ConA magnetic beads can efficiently and rapidly separate and purify various biomolecules such as polysaccharides, glycoproteins, and glycolipids by utilizing the affinity between ConA globulin A and the terminal α-D-mannose and α-D-glucose groups.\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003eConA magnetic beads offer a convenient method to separate or fix cells, ensuring minimal cell loss during subsequent washing steps. Additionally, they can be employed for the collection and fixation of nuclei. Moreover, these beads find utility in innovative techniques such as CUT \u0026amp; Run and CUT \u0026amp; Tag, which are revolutionary approaches used in ChIP-seq experiments.\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003eIn summary, ConA magnetic beads are powerful tools for the efficient purification of biomolecules, convenient cell separation and fixation, collection of nuclei, and application in cutting-edge experimental techniques..\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eSpecifications\u003c\/strong\u003e\u003c\/h3\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eCat.No.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e19810ES01 \/\u003cspan\u003e \u003c\/span\u003e19810ES03 \/\u003cspan\u003e \u003c\/span\u003e19810ES08 \/\u003cspan\u003e \u003c\/span\u003e19810ES20\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eSize\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e200 μL \/1 mL\/5 mL\/20 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cstrong\u003eCharacterastics\u003c\/strong\u003e\u003c\/h3\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eCharacteristics\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eDescription\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eProduct content\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e10 mg\/mL magnetic beads in specific protective buffer\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eCoupled protein\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eConcanavalin A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eCapacity\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e10\u003csup\u003e5\u003c\/sup\u003ecells\/μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eBeads size\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e1 μm\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eMagnetization\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eSuperparamagnetic\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eApplication\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eIsolating cells or glycoproteins,CUT\u0026amp;RUN,CUT\u0026amp;Tag\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eStorage buffer\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003ePBS (pH7.4), 0.01% Tween-20, 0.05% Proclin-300\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003ePerformance\u003cstrong\u003e\u003c\/strong\u003e\n\u003c\/h3\u003e\n\u003ctable cellspacing=\"0\" border=\"0\" class=\"MsoNormalTable\" style=\"margin-left: auto; margin-right: auto;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"239\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eBatch\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"247\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eInput cells\/mL\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"337\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eRemaining cells\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\/ml\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"313\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eCapture Rate\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd nowrap valign=\"center\" width=\"239\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eBlank\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd nowrap valign=\"bottom\" width=\"247\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e7.66E+06\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd nowrap valign=\"bottom\" width=\"337\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e7.66E+06\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd nowrap valign=\"center\" width=\"313\"\u003e\n\u003cp class=\"MsoNormal\" style=\"text-align: center;\"\u003e\u003cspan\u003e N\/A\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd nowrap valign=\"center\" width=\"239\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eB2901\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd nowrap valign=\"bottom\" width=\"247\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e7.66E+06\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd nowrap valign=\"bottom\" width=\"337\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e5.12E+04\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd nowrap valign=\"center\" width=\"313\"\u003e\n\u003cp align=\"right\" class=\"MsoNormal\" style=\"text-align: center;\"\u003e\u003cspan\u003e99.33%\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd nowrap valign=\"center\" width=\"239\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eB2901\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd nowrap valign=\"bottom\" width=\"247\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e7.66E+06\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd nowrap valign=\"bottom\" width=\"337\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e4.78E+04\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd nowrap valign=\"center\" width=\"313\"\u003e\n\u003cp align=\"right\" class=\"MsoNormal\" style=\"text-align: center;\"\u003e\u003cspan\u003e99.38%\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd nowrap valign=\"center\" width=\"239\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eB2902\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd nowrap valign=\"bottom\" width=\"247\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e7.66E+06\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd nowrap valign=\"bottom\" width=\"337\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e3.41E+04\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd nowrap valign=\"center\" width=\"313\"\u003e\n\u003cp style=\"text-align: center;\" align=\"right\" class=\"MsoNormal\"\u003e\u003cspan\u003e99.55%\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd nowrap valign=\"center\" width=\"239\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eB2902\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd nowrap valign=\"bottom\" width=\"247\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e7.66E+06\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd nowrap valign=\"bottom\" width=\"337\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cspan\u003e5.46E+04\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd nowrap valign=\"center\" width=\"313\"\u003e\n\u003cp align=\"right\" class=\"MsoNormal\" style=\"text-align: center;\"\u003e\u003cspan\u003e99.29%\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eTable 1. The Yeasen ConA beads have high capture rate. \u003c\/strong\u003eThe amount of Concanavalin A (ConA) used in proportion to the microspheres has been verified to ensure maximum binding capacity while preventing excessive aggregation of magnetic beads after cell binding in CUT\u0026amp;Tag experiments. For instance, using 10 μL of ConA-coated beads can bind a quantity of cells equivalent to E7 levels, with a binding efficiency exceeding 98%.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/bb_600x600.jpg?v=1719266332\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cstrong\u003eFigure 1. Yeasen ConA beads show high dispersity.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003eIn the bead labeling process, a non-biological sealant was chosen to ensure efficient closure without being affected by batch-to-batch variations. This guarantees excellent sealing of the magnetic beads, preserving their high monodispersity during storage of ConA-coated beads, which is beneficial for efficient binding to carbohydrate molecules in subsequent experiments.\u003c\/p\u003e\n\u003cp style=\"text-align: center;\"\u003e\u003cimg height=\"87\" width=\"628\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/beads.jpg?v=1719265487\" alt=\"\" style=\"display: block; margin-left: auto; margin-right: auto;\"\u003e\u003c\/p\u003e\n\u003cp style=\"font-weight: 400; text-align: center;\"\u003e\u003cstrong\u003eFigure 2.  CUT\u0026amp;Tag chromatin signal distribution using Yeasen Con A Beads.\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp style=\"font-weight: 400;\"\u003eThis product should be stored at 2~8℃ for 2 years.\u003c\/p\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong\u003eInstructions\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp style=\"font-weight: 400;\"\u003eThe following operations take purification of glycoproteins or isolation of cells as an example,As for\u003cspan\u003e \u003c\/span\u003eCUT\u0026amp;Tag\u003cspan\u003e \u003c\/span\u003eexperiments\u003cspan\u003e \u003c\/span\u003e, see Yeasen Cat#12598ES for protocol.\u003c\/p\u003e\n\u003ch4\u003e\u003cstrong\u003e1. Preparation of buffers\u003c\/strong\u003e\u003c\/h4\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eBuffer\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eComponents\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eBinding buffer\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e20 mM HEPES (pH7.5),\u003cspan\u003e \u003c\/span\u003e10 mM KCl,\u003cspan\u003e \u003c\/span\u003e1 mM CaCl\u003csub\u003e2\u003c\/sub\u003e,\u003cspan\u003e \u003c\/span\u003e1 mM MnCl\u003csub\u003e2\u003c\/sub\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eWash buffer\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e20 mM HEPES (pH7.5),\u003cspan\u003e \u003c\/span\u003e150 mM NaCl,\u003cspan\u003e \u003c\/span\u003e0.5 mM\u003cspan\u003e \u003c\/span\u003eSpermidine,\u003cspan\u003e \u003c\/span\u003e1×Protease inhibitors cocktail\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eElution buffer\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e5mM Tris (pH8.0), 150 mM NaCl, 1M Glucose\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e1）Required Materials Not Included：Magnetic Stand、Eppendorf tubes, PCR tubes, magnetic stands, thermocyclers etc.\u003c\/p\u003e\n\u003cp\u003e2）Equilibrate the Beads at room temperature for at least 30 min.Resuspend the beads thoroughly by vortexing or shaking the bottle.\u003c\/p\u003e\n\u003ch4\u003e\n\u003cstrong\u003e2. Sample handling（\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003etaking mammalian cells as an example\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003e）\u003c\/strong\u003e\n\u003c\/h4\u003e\n\u003cp\u003e1）Prepare mammalian cells(1.0×10\u003csup\u003e 4\u003c\/sup\u003e~1.0×10\u003csup\u003e 5\u003c\/sup\u003e cells), centrifuge (4℃，600×g, 3~5 min) and carefully\u003cspan\u003e \u003c\/span\u003ediscard the supernatant.\u003c\/p\u003e\n\u003cp\u003e2）Add 140 μL Binding buffer,mix well and resuspend the cells, centrifuge and collect (4℃，600×g, 3~5 min), carefully\u003cspan\u003e \u003c\/span\u003ediscard the supernatant.\u003c\/p\u003e\n\u003cp\u003e3）Add 90 μL Binding buffer,mix well and resuspend the cells.\u003cbr\u003eCaution:Tightly adherent mammalian cells can be obtained by partial digestion using an enzyme like trypsin. For animal tissues, plant cells, or fungal cells, obtaining dispersed cells or protoplasts often requires special treatments tailored to their specific characteristics.\u003c\/p\u003e\n\u003ch4\u003e\u003cstrong\u003e3. Prepare ConA beads\u003c\/strong\u003e\u003c\/h4\u003e\n\u003cp\u003e1）Gently pipette the ConA beads to fully suspension, place 10\u003cspan\u003e \u003c\/span\u003eμL of the beads suspension in a new 200 μL centrifuge tube.\u003c\/p\u003e\n\u003cp\u003e2）Add 40 μL Binding buffer, mix well and stand on the magnetic stand for 1 min and discard the supernatant after the magnetic beads adsorb to the side wall of the centrifuge tube.\u003c\/p\u003e\n\u003cp\u003e3）Add 10 μL Binding buffer, mix well and resuspend the ConA beads.\u003c\/p\u003e\n\u003ch4\u003e\u003cstrong\u003e4. Sample binding\u003c\/strong\u003e\u003c\/h4\u003e\n\u003cp\u003e1）Add the prepared cell sample to the pretreated beads, gently pipette the resuspended beads, then incubate on an inverted mixer (room temperature 30 min or 4℃ overnight).\u003c\/p\u003e\n\u003cp\u003e2）Stand on a magnetic stand for 1 min, wait for the magnetic beads to adsorb to the side wall of the centrifuge tube, carefully discard the supernatant.\u003c\/p\u003e\n\u003cp\u003e3）Add 500 μL Elution buffer to the cell-ConA beads complex and\u003cspan\u003e \u003c\/span\u003eGently pipette to\u003cspan\u003e \u003c\/span\u003eresuspend beads, then\u003cspan\u003e \u003c\/span\u003eStand on a magnetic stand for 1 min, wait for the magnetic beads to adsorb to the side wall of the centrifuge tube, carefully discard the supernatant.\u003c\/p\u003e\n\u003cp\u003e4）Repeat step 3) for three or four more times.\u003c\/p\u003e\n\u003ch4\u003e\u003cstrong\u003e5. Elution\u003c\/strong\u003e\u003c\/h4\u003e\n\u003cp\u003e1）For glycoproteins, add 50~250 μL Elution buffer, gently pipette the resuspended beads, then incubate on an inverted mixer (room temperature for10~30 min). After inverted mix,stand on a magnetic stand for 1 min, colect the supernatant to a new 1.5 mL tube to step SDS-PAGE or Western blot.\u003c\/p\u003e\n\u003cp\u003e2）For cell, no need to elution.\u003c\/p\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e1. Equilibrate the\u003cspan\u003e \u003c\/span\u003eConA beads at room temperature before use.\u003c\/p\u003e\n\u003cp\u003e2. Avoid freezing, otherwise degradate of the bead material or loss of activity.\u003c\/p\u003e\n\u003cp\u003e3. ConA requires the presence of Ca2+ and Mn2+ ions to be active, so reagents containing EDTA, or other metal ion chelators should be avoided during the experiment.\u003c\/p\u003e\n\u003cp\u003e4. When incubated with cells, ConA beads may aggregate, which is normal and does not affect the normal use of magnetic beads.\u003c\/p\u003e\n\u003cp\u003e5. This product is for research use only.\u003c\/p\u003e\n\u003cp\u003e6. Please operate with lab coats and disposable gloves，for your safety.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eDocuments:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eSafety Data Sheet\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/19810-MSDS-HB250826.pdf?v=1756261288\"\u003e19810_MSDS_HB250826_EN.PDF\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eManuals\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\" data-v-444b0c7e=\"\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/19810ES-Ver.EN20240219.pdf?v=1719506871\"\u003e19810_Manual_Ver.EN20240219.pdf\u003c\/a\u003e\u003c\/p\u003e\n                          \u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"200 μL","offer_id":53286019334509,"sku":"19810ES01","price":100.0,"currency_code":"USD","in_stock":true},{"title":"1 mL","offer_id":53286027919725,"sku":"19810ES03","price":400.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/bb_600x600_94c5825d-0e5d-4ac5-b842-52ab2a9d81c5.jpg?v=1777929287","url":"https:\/\/www.ebiohippo.com\/products\/concanavalin-a-coated-magnetic-beads-bhd20800001","provider":"BioHippo","version":"1.0","type":"link"}