{"product_id":"crabp2-antibody-cellular-retinoic-acid-binding-protein-2-bha17135750","title":"CRABP2 Antibody \/ Cellular retinoic acid-binding protein 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eCRABP2 Antibody \/ Cellular retinoic acid-binding protein 2 is a anti-CRABP2 Rabbit antibody Recombinant Rabbit Monoclonal clone 30C81 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e CRABP2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 30C81, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eCRABP2 antibody detects cellular retinoic acid-binding protein 2, encoded by the CRABP2 gene. Alternate names include CRABP-II, cellular retinoic acid-binding protein type II, and RA-binding protein 2. CRABP2 is a cytoplasmic protein that binds retinoic acid with high affinity, delivering it to the nucleus to interact with retinoic acid receptors (RARs). This shuttling mechanism ensures efficient retinoic acid signaling, which regulates cell differentiation, growth, and development. Structurally, CRABP2 belongs to the fatty acid-binding protein family, characterized by a beta-barrel fold that binds hydrophobic ligands.\u003cbr\u003e\u003cbr\u003eCRABP2 antibody is widely applied in developmental biology, cancer research, and dermatology. Retinoic acid is essential for embryonic patterning, limb development, and organogenesis, and CRABP2 ensures delivery of ligand to nuclear receptors. In skin, CRABP2 regulates keratinocyte differentiation and retinoid responses, linking it to dermatological therapies. In cancer biology, CRABP2 expression modulates sensitivity to retinoids, influencing proliferation and apoptosis.\u003cbr\u003e\u003cbr\u003eApplications of CRABP2 antibody include western blotting, immunohistochemistry, immunofluorescence, and ELISA. Western blotting detects CRABP2 protein in cell and tissue lysates, immunohistochemistry maps expression in embryonic and tumor tissues, and immunofluorescence highlights cytoplasmic and nuclear localization. These methods support research into retinoid signaling in diverse contexts.\u003cbr\u003e\u003cbr\u003eDysregulation of CRABP2 is linked to developmental abnormalities, cancer progression, and skin disease. Overexpression promotes retinoid sensitivity in some cancers, while downregulation confers resistance. By applying CRABP2 antibody, researchers can study how retinoid signaling contributes to disease and therapy response.\u003cbr\u003e\u003cbr\u003eCRABP2 interacts with nuclear RARs, cytoplasmic enzymes, and signaling molecules. This positions it as a central mediator of vitamin A biology.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=CRABP2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CRABP2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=CRABP2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/CRABP2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=CRABP2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047303569773,"sku":"FY12848","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_8879c668-c63a-489e-82f8-215736619e4a.jpg?v=1772019352","url":"https:\/\/www.ebiohippo.com\/products\/crabp2-antibody-cellular-retinoic-acid-binding-protein-2-bha17135750","provider":"BioHippo","version":"1.0","type":"link"}