{"product_id":"crbn-antibody-cereblon-bha17135349","title":"CRBN Antibody \/ Cereblon","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eCRBN Antibody \/ Cereblon is a anti-CRBN Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e CRBN\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eCRBN antibody recognizes Cereblon, a substrate receptor component of the CRL4 E3 ubiquitin ligase complex that plays a central role in targeted protein degradation. Cereblon binds to the DDB1-CUL4-ROC1 complex, recruiting specific substrates for ubiquitination and subsequent proteasomal degradation. This process regulates numerous cellular pathways, including cell cycle progression, metabolism, and neuronal function. The CRBN antibody is widely used to study protein homeostasis, drug-target interactions, and the mechanism of action of immunomodulatory drugs such as thalidomide, lenalidomide, and pomalidomide, which bind Cereblon to alter substrate specificity.\u003cbr\u003e\u003cbr\u003eCereblon is encoded by the CRBN gene located on human chromosome 3p26.2. The protein consists of 442 amino acids and contains a conserved Lon domain-like region essential for ligand binding and substrate recognition. Within the CRL4 complex, Cereblon acts as a molecular adapter, recruiting neo-substrates such as Ikaros (IKZF1), Aiolos (IKZF3), and CK1alpha in response to drug binding. This interaction forms the basis for therapeutic degradation of disease-associated transcription factors in multiple myeloma and other cancers. Loss-of-function mutations in CRBN have been linked to autosomal recessive intellectual disability, highlighting its importance in neuronal signaling and synaptic plasticity.\u003cbr\u003e\u003cbr\u003eThe CRBN antibody enables detailed examination of the protein's expression and regulation across tissues. Western blot analysis typically reveals bands near 51 kDa corresponding to full-length Cereblon, while smaller isoforms may be detected in certain tissues. Immunofluorescence shows localization to both the cytoplasm and nucleus, depending on cell type and activation state. Researchers use this antibody to assess CRBN abundance in studies of proteolysis-targeting chimeras (PROTACs), a rapidly growing class of therapeutic agents that harness Cereblon for selective degradation of target proteins.\u003cbr\u003e\u003cbr\u003eBeyond oncology, CRBN influences neuronal excitability, metabolic regulation, and circadian rhythm. It has been shown to modulate BK channel activity and AMP-activated protein kinase signaling, linking it to ion channel control and energy metabolism.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=CRBN - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CRBN - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=CRBN - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/CRBN - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=CRBN+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047288856941,"sku":"FY12446","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_4c916e21-1a92-494f-8965-a2e47ee0a140.jpg?v=1782237005","url":"https:\/\/www.ebiohippo.com\/products\/crbn-antibody-cereblon-bha17135349","provider":"BioHippo","version":"1.0","type":"link"}