{"product_id":"cre-and-gfp-expressing-rgd-fiber-modified-adenovirus-adrgd-gfp-icre-bhv21600347","title":"Cre and GFP expressing (RGD fiber modified) Adenovirus (Ad(RGD)-GFP-iCre)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAd(RGD)-GFP-iCre is a replication-defective recombinant Ad5 adenovirus expressing the GFP-iCre recombinase under the CMV promoter. It is used to deliver Cre activity to floxed alleles in cell lines and in vivo for conditional gene knockout, lineage tracing, and activation of Cre-dependent (DIO\/FLEX) reporters.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBackbone:\u003c\/strong\u003e Human adenovirus type 5 (Ad5) with E1 and E3 deleted (dE1\/E3). Replication-incompetent in standard cells; replication-competent helper cells (HEK293) are required for amplification.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePromoter (CMV):\u003c\/strong\u003e a strong, ubiquitous promoter active in most mammalian cell types.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTransgene:\u003c\/strong\u003e GFP-iCre (eGFP tag).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTiter \u0026amp; format:\u003c\/strong\u003e 1×10\u003csup\u003e10\u003c\/sup\u003e PFU\/ml in storage buffer (DMEM, 2% BSA, 2.5% glycerol or equivalent), supplied as a 200 µL aliquot.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eThe cell entry of adenovirus is dependent on the initial recognition of the coxsackie-adenovirus receptor (CAR) on the surafce, therefore the infection efficiency of adenovirus is very low for cells with low or no CAR receptor. An Arg-Gly-Asp (RGD) motif was introduced in the surface-exposed loop of the adenovirus fiber knob, this enables the adenovirus to bypass CAR and mediate cell entry via RGD binding integrins.\u003c\/p\u003e\u003cp\u003eRDG modified adenovirus can be used for “retargeting” cells that regular adenovirus doesn’t work well. The infection effeciency of RDG modified adenovirus can be tens or hundreds of times higher than standard Ad5 in some cells, including human or mouse macrophages and T cells etc.\u003c\/p\u003e\u003cp\u003eThis is a RGD modified replication-deficient adenovirus expressing codon optimized Cre and GFP under a CMV promoter. The Cre and GFP are separated by a 2A peptide.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eDecision-relevant for researchers studying Recombinases.\u003c\/li\u003e\n\u003cli\u003eAdenovirus-mediated delivery is well-established in primary cells, organoids, and small-animal models.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConditional gene knockout in floxed-allele cell lines and animals.\u003c\/li\u003e\n\u003cli\u003eLineage tracing using Cre-dependent reporter alleles.\u003c\/li\u003e\n\u003cli\u003eActivation of Cre-dependent (DIO\/FLEX) viral reporters or effectors.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eRecombination efficiency depends on Cre expression level, allele accessibility, and the time window assayed; allow 72–96 hours after infection for recombination to plateau.\u003c\/li\u003e\n\u003cli\u003eAdenoviral delivery is episomal and non-integrating; expression dilutes with cell division and typically lasts 1–2 weeks in dividing cells (longer in non-dividing cells such as hepatocytes, neurons, and cardiomyocytes).\u003c\/li\u003e\n\u003cli\u003ePre-existing anti-Ad5 neutralizing antibodies are common in human and primate hosts and can reduce in vivo transduction; this is less relevant in inbred laboratory mouse strains.\u003c\/li\u003e\n\u003cli\u003eMOI optimization is essential — over-dosing can cause cytopathic effects; under-dosing yields incomplete transduction. A 3–5× MOI titration in your specific cell or animal model is recommended.\u003c\/li\u003e\n\u003cli\u003eReplication-defective Ad5 vectors are typically handled at BSL-2; consult your institutional biosafety officer for specific transgenes and routes of use.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n  - NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n  - UniProt: https:\/\/www.uniprot.org\/\n  - Russell WC. Adenoviruses: update on structure and function. J Gen Virol 2009; 90:1–20.\n  - Alba R, Bosch A, Chillon M. Gutless adenovirus: last-generation adenovirus for gene therapy. Gene Ther 2005; 12 Suppl 1:S18–27.\n  - Sauer B, Henderson N. Site-specific DNA recombination in mammalian cells by the Cre recombinase of bacteriophage P1. Proc Natl Acad Sci USA 1988; 85:5166–70.\n  - Vendor reference: https:\/\/www.vectorbiolabs.com\/product\/1779-cre-and-gfp-expressing-rgd-fiber-modified-adenovirus\/\n--\u003e","brand":"Vector Biolabs","offers":[{"title":"1x10^10 PFU\/ml \/ 200 µL","offer_id":53286500401517,"sku":"1779","price":495.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/cre-and-gfp-expressing-rgd-fiber-modified-adenovirus-adrgd-gfp-icre-bhv21600347","provider":"BioHippo","version":"1.0","type":"link"}