{"product_id":"cre-bhn20152458","title":"Cre","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis GenCefe mRNA encodes \u003cstrong\u003eCre\u003c\/strong\u003e, a recombinase construct supplied for gene editing and genome engineering applications. The product is formulated as lyophilised, non-encapsulated RNA and is intended for use in cell-based research applications requiring transient protein expression with reduced immunogenicity.\u003c\/p\u003e\n\u003ch2\u003emRNA Construct Design\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003e5′ Cap:\u003c\/strong\u003e Cap1 (m7GpppNm) — co-transcriptionally added during in vitro transcription (IVT). Cap1 includes 2′-O-methylation at the first transcribed nucleotide, closely mimicking the cap structure found on endogenous mammalian mRNA and reducing recognition by innate immune sensors (e.g., IFIT1\/IFIT3).\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eModified Nucleotides:\u003c\/strong\u003e 100% N1-methylpseudouridine (m1Ψ; N1-Me-Pseudo UTP) substitution for all uridine residues. m1Ψ modification reduces TLR7\/TLR8-mediated innate immune activation and PKR-driven translational suppression, resulting in improved protein expression in immunocompetent cells and primary cell types.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003ePoly(A) Tail:\u003c\/strong\u003e 100–120 nt — enzymatically polyadenylated. The poly(A) tail stabilises the 3′ terminus, supports poly(A)-binding protein (PABP) recruitment, and enhances ribosome recycling for efficient cap-dependent translation.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003e5′ UTR:\u003c\/strong\u003e hHBA1 (hemoglobin subunit alpha 1 5′ UTR) — a well-characterised human UTR that supports efficient cap-dependent translation initiation.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003e3′ UTR:\u003c\/strong\u003e hHBA1 (hemoglobin subunit alpha 1 3′ UTR) — provides post-transcriptional stability and modulates mRNA decay kinetics.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSignal Peptide:\u003c\/strong\u003e No\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eProtein Tag:\u003c\/strong\u003e No\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eCodon Optimisation:\u003c\/strong\u003e No (native human codon usage retained)\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003emRNA Length:\u003c\/strong\u003e Provided upon order placement.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eForm:\u003c\/strong\u003e Lyophilised powder; reconstitute in DEPC-treated water as needed.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis mRNA is supplied as non-encapsulated, lyophilised powder. Delivery vehicle selection (LNP, electroporation, lipofection) is at the discretion of the end user and should be optimised for the target cell type and application.\u003c\/p\u003e\n\u003ch2\u003eBiological Background\u003c\/h2\u003e\n\u003cp\u003eSite-specific recombinases catalyse conservative DNA rearrangements between defined recombination sites, enabling precise excision, integration, or inversion of genetic elements without the requirement for homologous sequences. Cre recombinase, derived from bacteriophage P1, acts on 34-bp \u003cem\u003eloxP\u003c\/em\u003e recognition sequences to excise flanked (floxed) DNA segments or invert sequences in an orientation-dependent manner. Transient delivery of Cre as mRNA provides precise temporal control over recombination events, crucial for conditional gene knockout studies, lineage tracing experiments, and removal of selection cassettes in engineered cell lines, while avoiding the prolonged Cre expression associated with stable integrants that can cause cytotoxicity and off-target recombination at pseudo-\u003cem\u003eloxP\u003c\/em\u003e sites in the mammalian genome.\u003c\/p\u003e\n\u003ch2\u003eResearch Relevance and Current Trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eConditional gene knockout models: Cre mRNA enables precise temporal activation of floxed alleles in cell culture models without the leaky expression often observed with inducible Cre systems.\u003c\/li\u003e\n  \u003cli\u003eSelection marker removal: Cre mRNA-mediated excision of floxed antibiotic resistance or selection cassettes from engineered cell lines streamlines the generation of clean expression constructs.\u003c\/li\u003e\n  \u003cli\u003eLineage tracing in ex vivo systems: Transient Cre mRNA delivery to mixed cell populations initiates heritable reporter activation in Cre-responsive lineage reporter cells, enabling clonal tracking.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon Research Applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eFloxed allele activation — transient Cre mRNA delivery to cells carrying loxP-flanked conditional alleles to initiate gene knockout or reporter activation.\u003c\/li\u003e\n  \u003cli\u003eConstruct clean-up — mRNA Cre delivery to remove floxed selection markers or unwanted sequences from engineered cell lines without sustained Cre toxicity.\u003c\/li\u003e\n  \u003cli\u003eLineage tracing — Cre mRNA activation of Rosa26 or equivalent reporter alleles in mixed cell populations for clonal lineage marking.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eNotes for Experimental Interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eConfirm loxP site integrity in the target cell line before Cre mRNA delivery; partial loxP deletions or inversions may lead to incomplete or aberrant recombination outcomes.\u003c\/li\u003e\n  \u003cli\u003eCre mRNA expression is transient (peak at 12–24 h); in cells with low recombination efficiency, a second mRNA transfection 48 h later may improve allele conversion rates.\u003c\/li\u003e\n  \u003cli\u003eMonitor for off-target Cre toxicity: sustained or overexpressed Cre can cause chromosomal rearrangements at cryptic pseudo-loxP sites; use the minimum effective mRNA dose.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"GenCefe Biotech","offers":[{"title":"20 ug","offer_id":53239632265581,"sku":"IR0013002","price":279.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53239644553581,"sku":"IR0013010","price":339.0,"currency_code":"USD","in_stock":true},{"title":"200 ug","offer_id":53239644586349,"sku":"IR0013020","price":399.0,"currency_code":"USD","in_stock":true},{"title":"500 ug","offer_id":53239644619117,"sku":"IR0013050","price":459.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53239644651885,"sku":"IR0013100","price":549.0,"currency_code":"USD","in_stock":true},{"title":"5 mg","offer_id":53239644684653,"sku":"IR0013500","price":1799.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/mRNA_944f6581-770b-47e0-ad68-5326548f1001.png?v=1776677731","url":"https:\/\/www.ebiohippo.com\/products\/cre-bhn20152458","provider":"BioHippo","version":"1.0","type":"link"}