{"product_id":"crh-antibody-corticotropin-releasing-hormone-bha17136128","title":"CRH Antibody \/ Corticotropin Releasing Hormone","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eCRH Antibody \/ Corticotropin Releasing Hormone is a anti-CRH Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e CRH\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eCRH antibody detects Corticotropin releasing hormone, a neuropeptide hormone that regulates the hypothalamic-pituitary-adrenal (HPA) axis and stress response. The UniProt recommended name is Corticotropin releasing hormone (CRH). This 41-amino-acid peptide is synthesized in the hypothalamus and secreted into the hypophyseal portal circulation, where it stimulates adrenocorticotropic hormone (ACTH) release from the anterior pituitary.\u003cbr\u003e\u003cbr\u003eFunctionally, CRH antibody identifies the peptide precursor prepro-CRH, which undergoes proteolytic cleavage to produce the mature active hormone. CRH binds to G protein-coupled receptors CRHR1 and CRHR2 on pituitary corticotrophs, activating adenylate cyclase and cAMP-dependent signaling pathways that induce ACTH synthesis and secretion. ACTH in turn stimulates cortisol release from the adrenal cortex, completing the neuroendocrine feedback loop that maintains stress adaptation and homeostasis.\u003cbr\u003e\u003cbr\u003eThe CRH gene is located on chromosome 8q13.1 and is primarily expressed in the paraventricular nucleus of the hypothalamus, but also in peripheral tissues such as the placenta, adrenal glands, and gastrointestinal tract. CRH coordinates endocrine, autonomic, and behavioral responses to stress, linking neuroendocrine signaling with emotional and metabolic regulation.\u003cbr\u003e\u003cbr\u003ePathologically, dysregulated CRH expression is associated with anxiety, depression, Cushing�s syndrome, and inflammatory disorders. Overproduction of CRH leads to chronic activation of the HPA axis and elevated cortisol levels, while deficiency impairs stress response and adrenal function. Research using CRH antibody supports studies in neuroendocrinology, stress physiology, and behavioral neuroscience.\u003cbr\u003e\u003cbr\u003eCRH antibody is validated for immunohistochemistry, ELISA, and immunofluorescence to detect neuropeptides and hormones. \u003cbr\u003e\u003cbr\u003eStructurally, Corticotropin releasing hormone is a 41-residue peptide containing a disulfide bond that stabilizes its helical structure, essential for receptor binding and biological activity. Its precursor includes a signal peptide directing secretion and prohormone regions involved in processing. This antibody enables investigation of CRH's role in HPA axis regulation, neuropeptide signaling, and stress-related disorders.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=CRH - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CRH - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=CRH - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/CRH - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=CRH+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047319331181,"sku":"FY13226","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_99a9573e-3376-4fb2-82d3-51133b68a341.jpg?v=1782237084","url":"https:\/\/www.ebiohippo.com\/products\/crh-antibody-corticotropin-releasing-hormone-bha17136128","provider":"BioHippo","version":"1.0","type":"link"}