{"product_id":"crkl-antibody-crk-like-protein-bha17135734","title":"CrkL Antibody \/ Crk-like protein","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eCrkL Antibody \/ Crk-like protein is a anti-CrkL Rabbit antibody Recombinant Rabbit Monoclonal clone 32C63 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasmic, Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e CrkL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 32C63, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, IP, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eCrkL antibody detects Crk-like protein, encoded by the CRKL gene. Other identifiers include v-crk avian sarcoma virus CT10 oncogene homolog-like, adaptor protein CRKL, and CRK-like proto-oncogene. CrkL is a member of the Crk family of adaptor proteins that contain SH2 and SH3 domains. These modular domains mediate interactions with phosphorylated tyrosines and proline-rich motifs, enabling CrkL to assemble signaling complexes downstream of receptor tyrosine kinases, cytokine receptors, and integrins. By linking upstream receptors to downstream effectors, CrkL regulates processes such as cell adhesion, migration, proliferation, and survival.\u003cbr\u003e\u003cbr\u003eCrkL antibody is widely applied in cancer biology, immunology, and signal transduction research. CrkL is required for BCR-ABL signaling in chronic myeloid leukemia, where it mediates oncogenic transformation and survival. CrkL phosphorylation status serves as a biomarker for monitoring tyrosine kinase inhibitor therapy in leukemia patients. Beyond cancer, CrkL regulates immune receptor signaling, T cell activation, and integrin-mediated adhesion.\u003cbr\u003e\u003cbr\u003eApplications for CrkL antibody include western blotting, immunohistochemistry, immunofluorescence, and ELISA. Western blotting detects CrkL protein and phosphorylation isoforms, immunohistochemistry maps expression in tumors and lymphoid tissues, and immunofluorescence highlights localization at focal adhesions and signaling complexes. These methods provide comprehensive tools for analyzing CrkL biology in both normal and disease contexts.\u003cbr\u003e\u003cbr\u003eDysregulation of CrkL contributes to leukemia, solid tumors, and immune disorders. Overexpression and abnormal phosphorylation promote cell transformation, invasion, and therapy resistance. By applying CrkL antibody, researchers can evaluate CrkL as a diagnostic marker and therapeutic target. In development, CrkL supports neural crest migration and organogenesis, linking it to congenital malformations when disrupted.\u003cbr\u003e\u003cbr\u003eCrkL interacts with proteins including CBL, paxillin, p130CAS, and STAT5, orchestrating diverse signaling cascades. Antibody-based detection allows detailed dissection of these interactions across pathways.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=CrkL - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CrkL - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=CrkL - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/CrkL - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=CrkL+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047303274861,"sku":"FY12832","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_f5ccfe7c-45b1-418e-8b61-d7e85ce9cda0.jpg?v=1772019355","url":"https:\/\/www.ebiohippo.com\/products\/crkl-antibody-crk-like-protein-bha17135734","provider":"BioHippo","version":"1.0","type":"link"}