{"product_id":"csl-reporter-lentivirus-bhv19400215","title":"CSL Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eCSL, also known as CBF1 or RBP-Jκ, is the central DNA-binding transcription factor of the canonical Notch signaling pathway. In the absence of Notch activation, CSL functions as a transcriptional repressor at Notch target genes. Upon ligand binding, the Notch intracellular domain (NICD) is released and translocates to the nucleus, where it forms a ternary complex with CSL and Mastermind-like coactivators to activate transcription. Notch signaling, transmitted through Notch1-4 receptors, governs cell-fate decisions, differentiation, and tissue patterning throughout development, and its dysregulation contributes to cancer and developmental disorders. Because CSL integrates input from all four receptors, CSL reporters give a broad readout of pan-Notch activity.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe CSL Reporter Lentivirus is a transcription factor reporter system for robust detection of pan-Notch (Notch1-4) transcriptional activity in mammalian cells. Tandem Notch response elements, coupled to a minimal TATA promoter and upstream enhancer, drive a fluorescent or luminescent reporter in response to NICD-CSL complex formation. Unlike Notch1-preferential reporters, the CSL design captures a broader response, including signaling from the less potent Notch3. Stable lentiviral integration enables generation of polyclonal reporter cell lines for analysis by fluorescence microscopy, flow cytometry, or luminometry. The system is applied to study Notch signaling in development, differentiation, and cancer. Particles are purified by PEG precipitation and sucrose gradient centrifugation, supporting efficient transduction of difficult-to-transfect cells, including primary and cryopreserved cultures.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the CSL (CBF1\/RBP-Jk) transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251619160429,"sku":"LTV-0106-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53362032443757,"sku":"LTV-0106-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53362032476525,"sku":"LTV-0106-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53362032509293,"sku":"LTV-0106-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53362032542061,"sku":"LTV-0106-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53362032574829,"sku":"LTV-0106-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSL-Reporter-Fig2.jpg?v=1776934274","url":"https:\/\/www.ebiohippo.com\/products\/csl-reporter-lentivirus-bhv19400215","provider":"BioHippo","version":"1.0","type":"link"}