{"product_id":"ctsf-antibody-cathepsin-f-bha17136076","title":"CTSF Antibody \/ Cathepsin F","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eCTSF Antibody \/ Cathepsin F is a anti-CTSF Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS) with listed reactivity in Human. Reported localization: Cytoplasm (lysosome).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e CTSF\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eCTSF antibody detects Cathepsin F, a lysosomal cysteine protease that mediates protein degradation and turnover in endosomal and lysosomal compartments. The UniProt recommended name is Cathepsin F (CTSF). This protease belongs to the papain-like family of cysteine cathepsins involved in cellular protein recycling and immune antigen processing.\u003cbr\u003e\u003cbr\u003eFunctionally, CTSF antibody identifies a 484-amino-acid preproenzyme that undergoes proteolytic maturation into active heavy and light chains. Cathepsin F participates in degradation of intracellular and endocytosed proteins, contributing to antigen presentation via MHC class II molecules. It also influences apoptosis, tissue remodeling, and neurodegenerative processes through regulated proteolysis.\u003cbr\u003e\u003cbr\u003eThe CTSF gene is located on chromosome 11q13.1 and is expressed in brain, liver, spleen, and macrophages. Cathepsin F maintains lysosomal proteolytic balance and participates in innate immune defense through controlled protein breakdown and turnover.\u003cbr\u003e\u003cbr\u003ePathologically, CTSF mutations cause Kufs disease type B, an adult-onset neuronal ceroid lipofuscinosis characterized by progressive neurodegeneration. Dysregulated Cathepsin F expression has also been associated with cancer progression and inflammatory diseases. Research using CTSF antibody supports studies in lysosomal biology, protease regulation, and neurodegenerative mechanisms.\u003cbr\u003e\u003cbr\u003eCTSF antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect lysosomal proteases. \u003cbr\u003e\u003cbr\u003eStructurally, Cathepsin F features an N-terminal cystatin-like domain and a papain-like catalytic domain containing the conserved cysteine-histidine-asparagine catalytic triad. This antibody enables examination of CTSF's role in lysosomal degradation and neuronal maintenance.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=CTSF - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CTSF - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=CTSF - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/CTSF - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=CTSF+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047317856621,"sku":"FY13174","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_d2ce1857-33fb-4cc8-a616-6684c6108988.jpg?v=1782237101","url":"https:\/\/www.ebiohippo.com\/products\/ctsf-antibody-cathepsin-f-bha17136076","provider":"BioHippo","version":"1.0","type":"link"}