{"product_id":"ctsh-antibody-cathepsin-h-bha17135649","title":"CTSH Antibody \/ Cathepsin H","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eCTSH Antibody \/ Cathepsin H is a anti-CTSH Rabbit antibody Recombinant Rabbit Monoclonal clone 31C05 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e CTSH\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 31C05, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eCTSH antibody detects cathepsin H, a lysosomal cysteine protease encoded by the CTSH gene. Cathepsin H is also known as mini chain cathepsin H, lysosomal thiol protease H, and EC 3.4.22.16. As a member of the papain like protease family, cathepsin H plays a central role in intracellular protein degradation, antigen processing, and extracellular matrix turnover. The protein is synthesized as an inactive zymogen that is activated in the acidic environment of lysosomes. Structurally, cathepsin H contains a papain like catalytic domain and a mini chain insert that confers aminopeptidase activity in addition to endopeptidase function.\u003cbr\u003e\u003cbr\u003eCTSH antibody is applied in immunology, cancer biology, and developmental biology. Cathepsin H contributes to general protein turnover in lysosomes and is critical for remodeling of tissues during development and wound healing. In immune cells, CTSH processes antigens for presentation on MHC class II molecules, influencing adaptive immune responses. By detecting cathepsin H, researchers can investigate both housekeeping proteolysis and specialized immune functions.\u003cbr\u003e\u003cbr\u003e Western blot assays detect both procathepsin H and the mature active enzyme. Immunohistochemistry maps expression in tissues such as lung, kidney, and lymph nodes, while immunofluorescence highlights punctate lysosomal localization. These assays provide robust methods for assessing lysosomal function across systems.\u003cbr\u003e\u003cbr\u003eDysregulated CTSH activity has been implicated in cancer progression. Tumor cells exploit cathepsin H and related proteases to degrade extracellular matrix, promoting invasion and metastasis. Elevated expression of CTSH correlates with poor prognosis in lung and breast cancer. Conversely, cathepsin H deficiency impairs immune processing and contributes to altered inflammatory responses. By applying CTSH antibody, researchers can study the balance between protective immune function and pathological proteolysis.\u003cbr\u003e\u003cbr\u003eBeyond cancer, CTSH plays roles in metabolic and neurodegenerative disease. In the brain, altered lysosomal protease activity contributes to protein aggregation disorders such as Alzheimer disease. Cathepsin H has been linked to processing of amyloid precursor protein and clearance of aggregated proteins. In metabolic disorders, abnormal CTSH expression influences adipose tissue remodeling and insulin sensitivity.\u003cbr\u003e\u003cbr\u003eCTSH antibody also supports studies in developmental biology. Lysosomal proteases including cathepsin H are required for embryogenesis, bone remodeling, and organ morphogenesis. Mouse knockout models reveal roles in immune cell differentiation and growth factor activation. Detection of CTSH in these contexts informs both basic developmental mechanisms and potential therapeutic interventions.\u003cbr\u003e\u003cbr\u003eCTSH antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=CTSH - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CTSH - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=CTSH - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/CTSH - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=CTSH+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047298359661,"sku":"FY12746","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_c4fb7ea6-8369-40ac-8abc-05e2e908157b.jpg?v=1772019337","url":"https:\/\/www.ebiohippo.com\/products\/ctsh-antibody-cathepsin-h-bha17135649","provider":"BioHippo","version":"1.0","type":"link"}