{"product_id":"cxxc5-antibody-cxxc-type-zinc-finger-protein-5-bha17135381","title":"CXXC5 Antibody \/ CXXC-type zinc finger protein 5","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eCXXC5 Antibody \/ CXXC-type zinc finger protein 5 is a anti-CXXC5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e CXXC5\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eCXXC5 antibody recognizes CXXC-type zinc finger protein 5, a nuclear and cytoplasmic signaling regulator that serves as a negative feedback modulator of the Wnt\/beta-catenin pathway. CXXC5 belongs to the CXXC-type zinc finger family, whose members bind unmethylated CpG DNA and influence transcriptional regulation, epigenetic programming, and signal transduction. The CXXC5 antibody is commonly used in developmental biology, cancer, and neurobiology studies to analyze its role in cell differentiation, tissue regeneration, and signal modulation.\u003cbr\u003e\u003cbr\u003eCXXC5 is encoded by the CXXC5 gene located on human chromosome 5q31.2. The protein contains a CXXC-type zinc finger motif enabling DNA interaction, and a Dishevelled (DVL)-binding domain that directly inhibits Wnt signaling by preventing DVL-mediated beta-catenin stabilization. This feedback inhibition is essential for maintaining balanced Wnt activity during embryogenesis and adult tissue homeostasis. CXXC5 also acts as a transcriptional co-regulator by associating with DNA methylation machinery, including TET family enzymes, to maintain epigenetic stability.\u003cbr\u003e\u003cbr\u003eUsing the CXXC5 antibody, researchers can detect a 34-38 kilodalton protein by western blot and identify nuclear and cytoplasmic localization through immunofluorescence microscopy. CXXC5 expression is found in osteoblasts, endothelial cells, neurons, and fibroblasts, where it coordinates growth factor signaling and differentiation. In bone metabolism, CXXC5 inhibits osteoblast differentiation by suppressing Wnt signaling, while in the vascular system it regulates angiogenic responses. Its dysregulation contributes to conditions such as osteoporosis, impaired wound healing, and tumorigenesis.\u003cbr\u003e\u003cbr\u003eFunctionally, CXXC5 participates in feedback loops linking Wnt, bone morphogenetic protein, and transforming growth factor beta signaling pathways. It also affects neuronal growth cone development and synaptic plasticity. Recent studies suggest that pharmacologic disruption of the CXXC5-DVL interaction can enhance regenerative responses, including hair follicle regeneration and wound repair.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=CXXC5 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CXXC5 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=CXXC5 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/CXXC5 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=CXXC5+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047289610605,"sku":"FY12478","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_d4c9592d-aee5-4c69-a7b8-2ca99aa62c23.jpg?v=1782236998","url":"https:\/\/www.ebiohippo.com\/products\/cxxc5-antibody-cxxc-type-zinc-finger-protein-5-bha17135381","provider":"BioHippo","version":"1.0","type":"link"}