{"product_id":"cyp7b1-antibody-25-hydroxycholesterol-7-alpha-hydroxylase-bha17135697","title":"CYP7B1 Antibody \/ 25-hydroxycholesterol 7-alpha-hydroxylase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eCYP7B1 Antibody \/ 25-hydroxycholesterol 7-alpha-hydroxylase is a anti-CYP7B1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (ER).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e CYP7B1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eCYP7B1 antibody detects 25-hydroxycholesterol 7-alpha-hydroxylase, a cytochrome P450 enzyme that catalyzes the hydroxylation of oxysterols and steroids, contributing to bile acid synthesis and cholesterol homeostasis. Encoded by the CYP7B1 gene on chromosome 8q12.3, this microsomal monooxygenase regulates the alternative bile acid synthesis pathway by converting oxysterols such as 25-hydroxycholesterol and dehydroepiandrosterone (DHEA) into hydroxylated metabolites. CYP7B1 plays a key role in maintaining sterol balance and modulating neurosteroid and androgen metabolism.\u003cbr\u003e\u003cbr\u003eLocalized to the endoplasmic reticulum membrane, CYP7B1 uses NADPH-cytochrome P450 reductase as an electron donor for hydroxylation reactions. It is highly expressed in liver, brain, and steroidogenic tissues, where it contributes to both cholesterol catabolism and neuroactive steroid metabolism. By regulating oxysterol levels, CYP7B1 influences liver lipid homeostasis and protects against cholesterol accumulation and neurotoxicity.\u003cbr\u003e\u003cbr\u003eThe CYP7B1 antibody is widely used in metabolism, neurobiology, and endocrinology research to study bile acid biosynthesis, cholesterol turnover, and steroid regulation. Western blot analysis detects a 55 kilodalton band corresponding to CYP7B1, while immunofluorescence reveals endoplasmic reticulum localization in hepatocytes and neurons. This antibody provides a means to assess CYP7B1 expression under physiological and pathological conditions, including liver disease and metabolic disorders.\u003cbr\u003e\u003cbr\u003eMutations in CYP7B1 cause hereditary spastic paraplegia type 5A and neonatal cholestasis, demonstrating the enzyme's essential role in cholesterol detoxification and neuronal maintenance. Altered CYP7B1 expression has also been linked to atherosclerosis, Alzheimer's disease, and hormonal dysregulation. The CYP7B1 antibody supports research into cholesterol metabolism, neurosteroid synthesis, and disease pathophysiology.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=CYP7B1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=CYP7B1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=CYP7B1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/CYP7B1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=CYP7B1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047303668077,"sku":"FY12795","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_10c4cd3a-8198-4c66-b911-bb4cf020cbd7.jpg?v=1782237045","url":"https:\/\/www.ebiohippo.com\/products\/cyp7b1-antibody-25-hydroxycholesterol-7-alpha-hydroxylase-bha17135697","provider":"BioHippo","version":"1.0","type":"link"}