{"product_id":"danre-lmo2-antibody-rhombotin-2-bha17136430","title":"Danre Lmo2 Antibody \/ Rhombotin 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eDanre Lmo2 Antibody \/ Rhombotin 2 is a anti-LMO2 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Zebrafish. Reported localization: Nucleus, Cytoplasm, Cell surface.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e LMO2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit Ig\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eLmo2, or LIM domain only protein two, is a transcriptional regulator that plays a critical role in hematopoietic and vascular development. In zebrafish, Lmo2 is essential for the specification of blood and endothelial lineages and is widely used as a molecular marker for early hematopoietic progenitor cells.\u003cp\u003eLmo2 does not directly bind to DNA but functions as a scaffold protein, forming complexes with other transcription factors to regulate gene expression. During zebrafish embryogenesis, Lmo2 is expressed in the intermediate cell mass, the site of primitive blood cell formation, and in the developing vasculature. It is required for the emergence of both erythroid and myeloid lineages, as well as for the proper development of endothelial cells.\u003c\/p\u003e\n\u003cp\u003eLoss of Lmo2 function in zebrafish results in severe defects in blood cell formation and vascular patterning, highlighting its central role in mesodermal differentiation. Because of its strong evolutionary conservation and defined expression patterns, zebrafish Lmo2 serves as a powerful model for studying hematopoiesis, angiogenesis, and transcriptional networks involved in early development.\u003c\/p\u003e\n\u003cp\u003eLmo2 is also relevant to human health, as misregulation of Lmo2 has been associated with leukemia. This makes zebrafish Lmo2 a valuable tool in both basic research and disease modeling related to blood development and cancer biology.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=LMO2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=LMO2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=LMO2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/LMO2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=LMO2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047329259885,"sku":"RZ1235","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_4c2b7b14-c78f-466e-abb3-bc17441a5b30.jpg?v=1772019463","url":"https:\/\/www.ebiohippo.com\/products\/danre-lmo2-antibody-rhombotin-2-bha17136430","provider":"BioHippo","version":"1.0","type":"link"}