{"product_id":"dctpp1-antibody-dctp-pyrophosphatase-1-xtp3tpa-bha17135707","title":"DCTPP1 Antibody \/ dCTP pyrophosphatase 1 \/ XTP3TPA","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eDCTPP1 Antibody \/ dCTP pyrophosphatase 1 \/ XTP3TPA is a anti-DCTPP1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunohistochemistry (IHC), Western blot (WB) with listed reactivity in Human, Mouse. Reported localization: Cytoplasm, Nucleus.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e DCTPP1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e ELISA, FACS, IHC, WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eDCTPP1 antibody detects dCTP pyrophosphatase 1 (also known as XTP3TPA), a nucleotide pool sanitizing enzyme that prevents the incorporation of damaged or modified nucleotides into DNA. Encoded by the DCTPP1 gene on chromosome 16p13.3, this enzyme hydrolyzes dCTP and its oxidized derivatives to their monophosphate forms, maintaining genomic integrity by reducing mutagenic nucleotide incorporation. DCTPP1 plays an essential role in DNA replication fidelity, repair, and protection against oxidative stress-induced mutagenesis.\u003cbr\u003e\u003cbr\u003eLocated primarily in the nucleus and mitochondria, DCTPP1 acts as a homotetrameric hydrolase with substrate specificity for dCTP and 5-methyl-dCTP. By regulating the deoxynucleotide pool, it prevents mispairing and epigenetic instability caused by cytosine modifications. Its activity supports accurate DNA replication and limits the formation of base substitution mutations. DCTPP1 expression increases under oxidative stress and in proliferating cells to safeguard replication accuracy.\u003cbr\u003e\u003cbr\u003eThe DCTPP1 antibody is widely used in DNA metabolism, cancer, and oxidative stress research to investigate nucleotide sanitization, genome maintenance, and replication control. Western blot analysis detects a 21 kilodalton band corresponding to DCTPP1, while immunofluorescence reveals both nuclear and mitochondrial localization depending on cell type. This antibody enables researchers to explore DNA repair dynamics and nucleotide pool balance in diverse biological systems.\u003cbr\u003e\u003cbr\u003eLoss or dysregulation of DCTPP1 is associated with increased mutation rates, mitochondrial dysfunction, and genomic instability. Elevated expression has been observed in rapidly dividing tumor cells, where it supports proliferation by maintaining balanced nucleotide pools. The DCTPP1 antibody provides a powerful tool for studying DNA replication accuracy, oxidative defense, and cancer cell metabolism.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=DCTPP1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DCTPP1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=DCTPP1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/DCTPP1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=DCTPP1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047302455661,"sku":"FY12805","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_a7fc7db9-0598-4760-87e1-ef09bc232fe0.jpg?v=1782237056","url":"https:\/\/www.ebiohippo.com\/products\/dctpp1-antibody-dctp-pyrophosphatase-1-xtp3tpa-bha17135707","provider":"BioHippo","version":"1.0","type":"link"}