{"product_id":"ddddk-tag-antibody-flag-tag-bha17128270","title":"DDDDK Tag Antibody \/ FLAG Tag","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eDDDDK Tag Antibody \/ FLAG Tag is an antibody targeting \u003cstrong\u003eFLAG\u003c\/strong\u003e, raised in \u003cstrong\u003eRabbit\u003c\/strong\u003e for protein detection and localization studies where these specifications are required.\u003c\/p\u003e \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e FLAG.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eAntibody identity:\u003c\/strong\u003e Recombinant Rabbit Monoclonal; Clone RM399; Rabbit IgG.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eConjugate\/label:\u003c\/strong\u003e Unconjugated (affects detection chemistry and multiplex compatibility).\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Purified.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e DDDDK.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eListed applications:\u003c\/strong\u003e WB (refer to on-page specifications for application-specific guidance).\u003c\/li\u003e \u003c\/ul\u003e  \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eThis antibody reacts to recombinant proteins containing a DDDDK fused to either the amino or carboxy terminus. No cross reactivity with any endogenous protein.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eComparative expression profiling across cell types, tissues, or perturbations (e.g., drug treatment, genetic editing, or differentiation).\u003c\/li\u003e \u003cli\u003eSubcellular localization and trafficking studies, including co-localization with pathway markers in microscopy-based assays.\u003c\/li\u003e \u003cli\u003eIntegration of protein-level measurements with transcriptomics or proteomics to relate abundance to regulation and phenotype.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eWestern blotting: researchers commonly compare relative signal levels across conditions and use appropriate negative\/positive controls for interpretation.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eInterpretation should account for antibody-dependent factors such as epitope accessibility, isoforms, and sample preparation differences across workflows.\u003c\/p\u003e \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eIsoforms and PTMs:\u003c\/strong\u003e many targets have multiple isoforms and post-translational modifications that can shift apparent signal or localization; interpret bands\/signals accordingly.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eEpitope context:\u003c\/strong\u003e binding can depend on protein conformation and sample processing; region information in the title\/immunogen can help anticipate what may be detected.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eSpecies differences:\u003c\/strong\u003e predicted or validated reactivity may vary by ortholog sequence and sample context; confirm in your model system.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eControl concepts:\u003c\/strong\u003e include negative controls (no-primary\/isotype), and where possible genetic controls (KO\/KD) or independent antibodies to strengthen conclusions.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - UniProt search: FLAG — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=FLAG - Gene search: FLAG — NCBI Gene — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=FLAG - Ensembl search: FLAG — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=FLAG - PubMed search: FLAG antibody — PubMed — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=FLAG+antibody - Reactome search: FLAG — Reactome — https:\/\/reactome.org\/content\/query?q=FLAG --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"1 mg\/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide \/ 100 ug","offer_id":53046413918573,"sku":"R20415-100UG","price":439.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_ed67e89e-2bcb-43bc-97fb-0c0d8138692c.jpg?v=1772000514","url":"https:\/\/www.ebiohippo.com\/products\/ddddk-tag-antibody-flag-tag-bha17128270","provider":"BioHippo","version":"1.0","type":"link"}