{"product_id":"ddx4-antibody-bha17103577","title":"DDX4 Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eDDX4 antibody supplied as a purified reagent for WB in Human, Mouse samples. This product is a polyclonal (rabbit origin) antibody (host: Rabbit; isotype: Rabbit IgG) intended for research use only.\u003c\/p\u003e \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003e\n\u003cstrong\u003eAntibody identity:\u003c\/strong\u003e Polyclonal (rabbit origin); host Rabbit; isotype Rabbit IgG.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eFormat and purification:\u003c\/strong\u003e format: Purified; purity: Protein A affinity chromatography.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eSpecies reactivity (reported):\u003c\/strong\u003e Human, Mouse.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eApplications (listed):\u003c\/strong\u003e WB.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eImmunogen \/ epitope context:\u003c\/strong\u003e A recombinant protein fragment from the C-terminal region of DDX4\/VASA was used as the immunogen for the DDX4 antibody..\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eThese attributes help you align the antibody with the biological question (target state, sample type, and readout) while keeping interpretation grounded in appropriate controls.\u003c\/p\u003e \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eDDX4 is the intended antigen for this primary antibody. Reported biological context includes: DEAD box proteins are RNA helicases involved in cellular processes involving the alteration of RNA secondary structure. DDX4, DEAD box polypeptide 4, is the mammalian homolog of the Drosophila VASA gene.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eSpatial and single-cell approaches: imaging-based and cytometry workflows increasingly quantify heterogeneity and relocalization rather than only bulk abundance.\u003c\/li\u003e   \u003cli\u003eInteraction-centric biology: IP-based enrichment and proteomics are widely used to define complexes, binding partners, and context-specific interactomes.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eWestern blot (WB): compare relative abundance\/isoform patterns across conditions and sample types; band shifts may reflect processing or post-translational modification.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eAcross these readouts, differences in signal intensity, localization, or complex enrichment are typically interpreted alongside sample-matched controls and independent evidence to distinguish regulation from technical variation.\u003c\/p\u003e \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eIsoforms, cleavage products, or post-translational modifications can alter apparent molecular weight and subcellular distribution; interpret bands and staining patterns in the context of expected biology and sample preparation.\u003c\/li\u003e   \u003cli\u003eSpecies differences and epitope conservation may affect binding; use matched positive controls and orthogonal evidence when comparing across organisms.\u003c\/li\u003e   \u003cli\u003eControl concepts: include appropriate isotype and secondary-only controls (for imaging), and consider genetic perturbations (knockout\/knockdown\/overexpression) or independent antibodies targeting distinct epitopes to strengthen conclusions.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eEpitope context is defined by the immunogen description; when available, align this with known domains, PTM sites, or family homology to anticipate potential cross-reactivity patterns. As a polyclonal antibody, recognition spans multiple epitopes, which can improve detection across conformations but may broaden background depending on sample context.\u003c\/p\u003e \u003c!-- Sources (internal): - NCBI Gene search (DDX4) — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DDX4 - Ensembl search (DDX4) — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=DDX4 - PubMed search (DDX4) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=DDX4 - Reactome pathway search (DDX4) — Reactome — https:\/\/reactome.org\/content\/query?q=DDX4 --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5 mg\/ml in 1X PBS with 0.1 mg\/ml BSA (US sourced), 0.05% sodium azide \/ 100 ug","offer_id":53043244138861,"sku":"N1195-100UG","price":439.0,"currency_code":"USD","in_stock":true},{"title":"0.5 mg\/ml in 1X PBS with 0.1 mg\/ml BSA (US sourced), 0.05% sodium azide \/ 25 ug","offer_id":53043672088941,"sku":"N1195-25UG","price":225.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_b9736e8d-0ea0-40cd-bd44-2dffe31bdb15.jpg?v=1771934378","url":"https:\/\/www.ebiohippo.com\/products\/ddx4-antibody-bha17103577","provider":"BioHippo","version":"1.0","type":"link"}