{"product_id":"dectin1-nf-kappa-b-reporter-lentivirus-bhv19400266","title":"DECTIN1\/NFκB Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eDectin-1 is a C-type lectin receptor expressed on dendritic cells, macrophages, neutrophils, and other myeloid cells, where it serves as a key pattern recognition receptor of the innate immune system. It recognizes beta-glucans, carbohydrate components of fungal cell walls, and signals through its immunoreceptor tyrosine-based activation-like motif and the Syk kinase pathway. This signaling activates the transcription factor NF-κB, driving the production of pro-inflammatory cytokines, reactive oxygen species, and other effectors of antifungal defense, and helping to shape adaptive immunity, including Th17 responses. Dectin-1 is studied for its central role in antifungal immunity and inflammation and as a target for immunomodulatory and vaccine adjuvant strategies.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe DECTIN1\/NF-κB Reporter Lentivirus is an immunotherapy reporter system supplied as a two-vial set. A receptor lentivirus drives constitutive expression of human Dectin-1 under a constitutive promoter with antibiotic selection, while a reporter lentivirus carries tandem NF-κB response elements driving a dual reporter combining GFP and secreted Gaussia luciferase. Sequential transduction and selection generates a dual-stable reporter cell line that responds quantitatively to Dectin-1 engagement, for example by fungal beta-glucans, with a combined fluorescent and bioluminescent readout. Secreted Gaussia luciferase accumulates in conditioned media for kinetic sampling without cell lysis. Supplied as high-titer, VSV-G pseudotyped third-generation particles purified by PEG precipitation and sucrose gradient centrifugation, the system supports studies of antifungal immunity, inflammatory signaling, and Dectin-1-targeting therapeutics in primary and difficult-to-transfect cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis 2-vial immunotherapy reporter system consists of a \u003cstrong\u003eVial 1 Receptor Lentivirus\u003c\/strong\u003e encoding human DECTIN1 under a constitutive promoter with antibiotic selection, and a \u003cstrong\u003eVial 2 Reporter Lentivirus\u003c\/strong\u003e encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (GFP, GFP-P2A-GLuc, GLuc, GLuc-P2A-GFP, GLuc-P2A-RFP, RFP, RFP-P2A-GLuc). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.\u003c\/p\u003e\n\u003cp\u003eSecreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GLuc-P2A-GFP \/ Blasticidin \/ 2x10^6","offer_id":53251616080237,"sku":"TRV-0018-6S","price":895.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Vector-Layout-1024x261_f2fbdbb6-e963-44e8-8ceb-510895cdf85d.jpg?v=1782157779","url":"https:\/\/www.ebiohippo.com\/products\/dectin1-nf-kappa-b-reporter-lentivirus-bhv19400266","provider":"BioHippo","version":"1.0","type":"link"}