{"product_id":"deptor-antibody-depdc6-bha17135872","title":"DEPTOR Antibody \/ DEPDC6","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eDEPTOR Antibody \/ DEPDC6 is a anti-DEPTOR Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (lysosome).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e DEPTOR\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eDEPTOR antibody detects DEP domain-containing mTOR-interacting protein, an endogenous inhibitor of the mTORC1 and mTORC2 complexes. The UniProt recommended name is DEP domain-containing mTOR-interacting protein (DEPTOR), also known as DEP domain-containing protein 6 (DEPDC6). DEPTOR acts as a critical regulator of cell growth, metabolism, and survival by directly binding to mechanistic target of rapamycin (mTOR), suppressing its kinase activity.\u003cbr\u003e\u003cbr\u003eFunctionally, DEPTOR antibody recognizes a 409-amino-acid cytoplasmic protein that negatively regulates mTOR signaling. DEPTOR binds to both mTORC1 and mTORC2 via its PDZ domain, preventing phosphorylation of downstream effectors such as S6K1 and AKT. Its expression is finely tuned by nutrient availability, growth factors, and stress stimuli. Under normal conditions, mTOR phosphorylates DEPTOR, marking it for ubiquitination and proteasomal degradation, thereby sustaining mTOR activity. Conversely, nutrient deprivation or stress increases DEPTOR levels, suppressing mTOR signaling to promote autophagy and energy conservation.\u003cbr\u003e\u003cbr\u003eThe DEPTOR gene is located on chromosome 8q24.12 and encodes a DEP and PDZ domain-containing protein that integrates environmental cues with cellular metabolic state. DEPTOR functions as a feedback regulator ensuring mTOR pathway balance. Dysregulation of DEPTOR disrupts this feedback loop, leading to aberrant cell growth and metabolic reprogramming. In cancer, overexpression of DEPTOR has been observed in multiple myeloma, where it paradoxically supports survival by maintaining AKT activation through mTORC2 inhibition.\u003cbr\u003e\u003cbr\u003eDEPTOR antibody is used to explore cellular signaling pathways involving mTOR, autophagy, and nutrient sensing. It supports applications such as western blotting, immunoprecipitation, and fluorescence microscopy to monitor DEPTOR expression and mTOR complex association. In metabolic studies, DEPTOR serves as a marker for energy stress adaptation, linking insulin signaling and amino acid availability to cell growth control. Elevated DEPTOR expression modulates lipid biosynthesis and glucose uptake pathways, reinforcing its role in metabolic regulation.\u003cbr\u003e\u003cbr\u003eStructurally, DEPTOR contains an N-terminal DEP domain responsible for membrane association and a C-terminal PDZ domain mediating protein-protein interactions with mTOR and associated complexes. Post-translational modifications including phosphorylation and ubiquitination govern its stability and turnover. DEPTOR also interacts with transcription factors and kinases outside the mTOR axis, expanding its regulatory network.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=DEPTOR - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DEPTOR - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=DEPTOR - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/DEPTOR - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=DEPTOR+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047307436397,"sku":"FY12970","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_23430f3c-d371-462d-826a-961293836c3e.jpg?v=1782237062","url":"https:\/\/www.ebiohippo.com\/products\/deptor-antibody-depdc6-bha17135872","provider":"BioHippo","version":"1.0","type":"link"}