{"product_id":"dhx58-antibody-dexh-box-helicase-58-bha17135550","title":"DHX58 Antibody \/ DExH-box helicase 58","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eDHX58 Antibody \/ DExH-box helicase 58 is a anti-DHX58 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e DHX58\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eDHX58 antibody detects DExH-box helicase 58, also known as LGP2, a cytoplasmic RNA helicase that functions as a regulator of antiviral innate immune responses. DHX58 belongs to the RIG-I-like receptor (RLR) family, which includes RIG-I and MDA5, and is involved in sensing viral RNA to trigger interferon production. The DHX58 antibody is widely used in immunology, virology, and innate signaling research to study viral RNA recognition and interferon pathway regulation.\u003cbr\u003e\u003cbr\u003eDHX58 is encoded by the DHX58 gene located on human chromosome 17q21.1. The protein is approximately 678 amino acids long and contains a helicase core domain, ATP-binding motifs, and a C-terminal regulatory domain. Unlike RIG-I and MDA5, DHX58 lacks the N-terminal CARD domains required for direct signaling, functioning instead as a modulator of their activity. It localizes to the cytoplasm and associates with stress granules and viral replication complexes during infection.\u003cbr\u003e\u003cbr\u003eThe DHX58 antibody detects a 75 kilodalton band by western blot and exhibits punctate cytoplasmic staining by immunofluorescence microscopy. DHX58 acts as a positive or negative regulator of antiviral signaling depending on context. It binds double-stranded RNA and modulates the activity of MDA5 and RIG-I, fine-tuning the magnitude and duration of type I interferon responses. During viral infection, DHX58 helps discriminate self from non-self RNA, preventing inappropriate immune activation.\u003cbr\u003e\u003cbr\u003eMutations or altered expression of DHX58 affect susceptibility to viral pathogens such as influenza, hepatitis C, and coronaviruses. It also influences autoimmunity, where excessive activation of the RLR pathway leads to chronic inflammation and tissue damage. Beyond immunity, DHX58 participates in RNA metabolism and stress granule dynamics, linking it to translational control during cellular stress.\u003cbr\u003e\u003cbr\u003eAs a key regulator of antiviral sensing, DHX58 provides a molecular handle for understanding innate immune balance and pathogen recognition.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=DHX58 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DHX58 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=DHX58 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/DHX58 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=DHX58+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047294919021,"sku":"FY12647","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_494f8484-9c55-4697-a404-291ec6ea5b4d.jpg?v=1782237029","url":"https:\/\/www.ebiohippo.com\/products\/dhx58-antibody-dexh-box-helicase-58-bha17135550","provider":"BioHippo","version":"1.0","type":"link"}