{"product_id":"dio2-antibody-type-ii-iodothyronine-deiodinase-bha17135295","title":"DIO2 Antibody \/ Type II iodothyronine deiodinase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eDIO2 Antibody \/ Type II iodothyronine deiodinase is a anti-DIO2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB) with listed reactivity in Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e DIO2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe DIO2 antibody targets Type II iodothyronine deiodinase, an enzyme encoded by the DIO2 gene that catalyzes the conversion of thyroxine (T4) to the active thyroid hormone triiodothyronine (T3). Type II iodothyronine deiodinase plays a central role in maintaining local and systemic thyroid hormone homeostasis by regulating intracellular T3 availability. This selenoenzyme is expressed in the brain, pituitary, brown adipose tissue, and skeletal muscle, where it enables tissue-specific modulation of thyroid hormone signaling. The DIO2 antibody provides a robust tool for studying thyroid hormone metabolism and energy regulation.\u003cbr\u003e\u003cbr\u003eType II iodothyronine deiodinase is a homodimeric membrane-bound enzyme located in the endoplasmic reticulum, characterized by a conserved selenocysteine residue within its catalytic center. It activates prohormone T4 to T3 by inner-ring deiodination, enhancing thyroid hormone receptor activation in target cells. The DIO2 antibody enables visualization of this enzyme in thyroid hormone–responsive tissues, facilitating studies of local hormone activation under varying physiological conditions such as cold exposure, fasting, or inflammation.\u003cbr\u003e\u003cbr\u003eRegulation of DIO2 is complex, involving both transcriptional and post-translational control. Its expression increases in response to sympathetic stimulation, especially in brown adipose tissue, where it supports thermogenesis by elevating mitochondrial metabolism. The DIO2 antibody supports research into these adaptive responses, helping elucidate mechanisms that coordinate hormonal signaling and energy expenditure. In the central nervous system, DIO2 provides local T3 for neurons and glial cells, influencing neurodevelopment and hypothalamic feedback regulation of thyroid-stimulating hormone (TSH).\u003cbr\u003e\u003cbr\u003eMutations or polymorphisms in the DIO2 gene have been linked to thyroid disease, insulin resistance, and altered metabolic rate. Abnormal DIO2 activity may contribute to hypothyroidism, obesity, and neuropsychiatric disorders. The DIO2 antibody allows quantification of enzyme expression in tissues and model systems used to study endocrine and metabolic diseases. Detection of Type II iodothyronine deiodinase also aids in assessing how thyroid hormone activation varies among tissues under different metabolic states.\u003cbr\u003e\u003cbr\u003eThe DIO2 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry. It provides strong cytoplasmic staining consistent with endoplasmic reticulum localization.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=DIO2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DIO2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=DIO2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/DIO2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=DIO2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047286464877,"sku":"FY12392","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_c4b49710-5a41-4271-8b0c-90a6077b2642.jpg?v=1782237003","url":"https:\/\/www.ebiohippo.com\/products\/dio2-antibody-type-ii-iodothyronine-deiodinase-bha17135295","provider":"BioHippo","version":"1.0","type":"link"}