{"product_id":"dis3-antibody-exosome-complex-exonuclease-rrp44-bha17136121","title":"DIS3 Antibody \/ Exosome complex exonuclease RRP44","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eDIS3 Antibody \/ Exosome complex exonuclease RRP44 is a anti-DIS3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western blot (WB) with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e DIS3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e ELISA, FACS, IF, IHC, ICC, WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eDIS3 antibody detects Exosome complex exonuclease RRP44, a catalytic subunit of the RNA exosome complex responsible for RNA degradation and processing. The UniProt recommended name is Exosome complex exonuclease RRP44 (DIS3). This enzyme functions as both a 3'-5' exoribonuclease and endoribonuclease, ensuring precise control of RNA quality, turnover, and surveillance in eukaryotic cells.\u003cbr\u003e\u003cbr\u003eFunctionally, DIS3 antibody identifies a 958-amino-acid cytoplasmic and nuclear protein that associates with the multi-protein RNA exosome complex. DIS3 degrades defective, misprocessed, or surplus RNA species, including rRNA, snoRNA, snRNA, and mRNA. It possesses two catalytic domains�an RNB domain for exonucleolytic degradation and a PIN domain for endonucleolytic cleavage�allowing versatile RNA processing under various physiological conditions. By maintaining RNA homeostasis, DIS3 contributes to normal gene expression and cellular viability.\u003cbr\u003e\u003cbr\u003eThe DIS3 gene is located on chromosome 13q22.1 and is expressed ubiquitously in proliferative tissues. Its activity is regulated through complex assembly, post-translational modification, and subcellular localization. DIS3 operates in both the nucleus, where it processes pre-rRNA and small noncoding RNAs, and the cytoplasm, where it degrades aberrant mRNA species.\u003cbr\u003e\u003cbr\u003ePathologically, mutations in DIS3 cause multiple myeloma, acute myeloid leukemia, and related hematologic malignancies. These mutations impair RNA degradation, leading to accumulation of defective transcripts and deregulated gene expression. Loss of DIS3 function disrupts RNA quality control and contributes to oncogenic transformation. Research using DIS3 antibody supports studies in RNA metabolism, gene regulation, and cancer genomics.\u003cbr\u003e\u003cbr\u003eDIS3 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect exosome complex components. \u003cbr\u003e\u003cbr\u003eStructurally, Exosome complex exonuclease RRP44 contains an N-terminal PIN domain for endonucleolytic activity and a central RNB catalytic core typical of RNase II family enzymes. The C-terminal S1 and CSD domains enable RNA substrate recognition and binding. This antibody aids in investigating DIS3's role in RNA exosome-mediated decay and RNA quality control in normal and malignant cells.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=DIS3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DIS3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=DIS3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/DIS3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=DIS3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047319101805,"sku":"FY13219","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_a37b0486-a91f-45b5-8d3b-b42b0bbe7817.jpg?v=1772019455","url":"https:\/\/www.ebiohippo.com\/products\/dis3-antibody-exosome-complex-exonuclease-rrp44-bha17136121","provider":"BioHippo","version":"1.0","type":"link"}