{"product_id":"dkc1-antibody-dyskerin-bha17136081","title":"DKC1 Antibody \/ Dyskerin","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eDKC1 Antibody \/ Dyskerin is a anti-DKC1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nucleus, Nucleolus, Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e DKC1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eDKC1 antibody detects Dyskerin, a nucleolar pseudouridine synthase essential for ribosomal RNA modification, telomerase stability, and small nucleolar RNP biogenesis. The UniProt recommended name is Dyskerin (DKC1). This enzyme catalyzes the isomerization of uridine to pseudouridine in rRNA and snRNA, ensuring ribosome maturation and translation fidelity.\u003cbr\u003e\u003cbr\u003eFunctionally, DKC1 antibody identifies a 514-amino-acid protein localized in the nucleolus and Cajal bodies. DKC1 associates with NOP10, NHP2, and GAR1 to form the H\/ACA ribonucleoprotein complex that modifies rRNA and snRNA. It also binds the telomerase RNA component (TERC), stabilizing the telomerase complex for telomere elongation.\u003cbr\u003e\u003cbr\u003eThe DKC1 gene is located on chromosome Xq28 and is ubiquitously expressed in dividing and metabolically active cells. Dyskerin function is vital for maintaining ribosome synthesis, RNA quality control, and telomere homeostasis. Its activity directly influences cell growth and chromosomal stability.\u003cbr\u003e\u003cbr\u003ePathologically, mutations in DKC1 cause X-linked dyskeratosis congenita, a rare disorder characterized by bone marrow failure, premature aging, and cancer predisposition. Reduced DKC1 function leads to defective rRNA modification, impaired telomerase activity, and genomic instability. Research using DKC1 antibody supports studies in RNA processing, telomere biology, and genetic diseases.\u003cbr\u003e\u003cbr\u003eDKC1 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect pseudouridine synthases and nucleolar proteins. \u003cbr\u003e\u003cbr\u003eStructurally, Dyskerin features a TruB-like catalytic domain with conserved aspartate essential for pseudouridylation activity, and a nuclear localization signal directing nucleolar import. This antibody aids in elucidating DKC1's role in ribonucleoprotein assembly and RNA modification fidelity.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=DKC1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DKC1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=DKC1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/DKC1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=DKC1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047314514285,"sku":"FY13179","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_111138d4-d4dc-46bf-9e1d-82275e4586b1.jpg?v=1782237111","url":"https:\/\/www.ebiohippo.com\/products\/dkc1-antibody-dyskerin-bha17136081","provider":"BioHippo","version":"1.0","type":"link"}