{"product_id":"dl5000-dna-marker-bhn15200002","title":"DL5000 DNA Marker","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}.bhp-desc .bhp-comp-table th{width:auto}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eDNA Ladders \u0026amp; Markers\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eDL5000 DNA Marker (DL 5000 Ladder DNA Marker) is composed of 8 kinds of DNA fragments with a length of 100 bp to 5,000 bp, dissolved in 1× Loading Buffer, and can be loaded directly, 5–10 µL per well, with no further preparation.\u003c\/p\u003e\n\u003cp\u003eThe eight fragments are 100, 250, 500, 750, 1,000, 2,000, 3,000 and 5,000 bp. The 750 bp and 3,000 bp reference bands contain 100 ng per 5 µL and every other band contains 50 ng per 5 µL. The marker is ready to use and needs no added loading buffer. For fragments of 100–500 bp a 2.5%–3% agarose gel is recommended, and for fragments of 500–5,000 bp a 1.5%–2% agarose gel is recommended.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFragment range\u003c\/th\u003e\n\u003ctd\u003e100–5,000 bp\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eNumber of bands\u003c\/th\u003e\n\u003ctd\u003e8\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBand sizes\u003c\/th\u003e\n\u003ctd\u003e100, 250, 500, 750, 1,000, 2,000, 3,000 and 5,000 bp\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReference band(s)\u003c\/th\u003e\n\u003ctd\u003e750 bp and 3,000 bp at 100 ng per 5 µL; other bands 50 ng per 5 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003eReady-to-use; premixed in 1× Loading Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eRecommended load\u003c\/th\u003e\n\u003ctd\u003e5–10 µL per well; 5 µL for wells narrower than 5 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eRecommended gel\u003c\/th\u003e\n\u003ctd\u003e2.5%–3% agarose for 100–500 bp; 1.5%–2% agarose for 500–5,000 bp\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eSizing of DNA fragments in agarose gel electrophoresis\u003c\/li\u003e\n\u003cli\u003eDirect loading as a size reference alongside samples\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKey Features\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003eTwo optimized Loading Buffer dyes are added without blocking the brightness of DNA band fluorescence.\u003c\/li\u003e\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eStored at -20°C with a shelf life of two years.\u003c\/p\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eLoad 5 µL per run when the sample well is narrower than 5 mm; increase the volume for wider wells.\u003c\/li\u003e\n\u003cli\u003eUse 2.5%–3% agarose for fragments expected between 100 and 500 bp, and 1.5%–2% agarose for fragments expected between 500 and 5,000 bp.\u003c\/li\u003e\n\u003cli\u003eAgarose purity strongly affects the clarity of DNA bands, so the best quality agarose should be used.\u003c\/li\u003e\n\u003cli\u003eHigher agarose concentration improves separation of short DNA fragments, while lower concentration favours separation of long fragments.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eFor research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.\u003c\/p\u003e\u003c\/div\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eGrade\u003c\/strong\u003eResearch Use Only\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eManufacturer\u003c\/strong\u003eELK Biotechnology\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eCatalog No.\u003c\/strong\u003eEDL102\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eBioHippo SKU\u003c\/strong\u003eBHN15200002\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"500 uL","offer_id":53965366657389,"sku":"EDL102-500UL","price":0.0,"currency_code":"USD","in_stock":true},{"title":"5 mL","offer_id":53965366788461,"sku":"EDL102-5ML","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EDL102-a-1.jpg?v=1789414949","url":"https:\/\/www.ebiohippo.com\/products\/dl5000-dna-marker-bhn15200002","provider":"BioHippo","version":"1.0","type":"link"}