{"product_id":"dna-topoisomerase-dna-decatenation-assay-kit-bht16300083","title":"DNA Topoisomerase DNA Decatenation Assay Kit","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ol,.bhp-desc ul{margin:0 0 12px 20px}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eDrug Target Assay Kit\u003c\/span\u003e\u003cp\u003eDuring DNA replication, bacterial DNA topoisomerase IV (the ParC-ParE complex) converts concatenated, or interlinked, DNA into decatenated DNA. Some decatenation activity is also seen with other topoisomerases, such as topoisomerase I and bacterial gyrases.\u003c\/p\u003e\n\u003ch2\u003eFormats and Pack Sizes\u003c\/h2\u003e\n\u003cp\u003eDDC020K contains the buffer, concatenated DNA, ATP, EDTA, dye and 20 spin columns but no enzyme (bacterial topoisomerase IV is supplied separately). DDC020KE adds 12 µl of 100 x E. coli topoisomerase IV (500 nM) for 20 assays, and DDC100KE is the 100-assay size with E. coli topoisomerase IV included and volumes scaled up.\u003c\/p\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eKit (enzyme not included), 20 assays (DDC020K)\u003c\/th\u003e\n\u003ctd\u003e20 assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Plus (E. coli Topo IV included), 20 assays (DDC020KE)\u003c\/th\u003e\n\u003ctd\u003e20 assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Plus (E. coli Topo IV included), 100 assays (DDC100KE)\u003c\/th\u003e\n\u003ctd\u003e100 assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eKit (enzyme not included), 20 assays (DDC020K)\u003c\/th\u003e\n\u003cth\u003eKit Plus (E. coli Topo IV included), 20 assays (DDC020KE)\u003c\/th\u003e\n\u003cth\u003eKit Plus (E. coli Topo IV included), 100 assays (DDC100KE)\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAssay buffer (Buffer T4)\u003c\/td\u003e\n\u003ctd\u003e150 µl (10 x)\u003c\/td\u003e\n\u003ctd\u003e150 µl (10 x)\u003c\/td\u003e\n\u003ctd\u003e600 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10 x Concatenated DNA\u003c\/td\u003e\n\u003ctd\u003e105 µl\u003c\/td\u003e\n\u003ctd\u003e105 µl\u003c\/td\u003e\n\u003ctd\u003e520 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e50 x ATP (10 mM)\u003c\/td\u003e\n\u003ctd\u003e30 µl\u003c\/td\u003e\n\u003ctd\u003e30 µl\u003c\/td\u003e\n\u003ctd\u003e120 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e0.4 M EDTA\u003c\/td\u003e\n\u003ctd\u003e120 µl\u003c\/td\u003e\n\u003ctd\u003e120 µl\u003c\/td\u003e\n\u003ctd\u003e550 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e20 x Fluorescence dye\u003c\/td\u003e\n\u003ctd\u003e160 µl\u003c\/td\u003e\n\u003ctd\u003e160 µl\u003c\/td\u003e\n\u003ctd\u003e780 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSpin columns\u003c\/td\u003e\n\u003ctd\u003e20 columns\u003c\/td\u003e\n\u003ctd\u003e20 columns\u003c\/td\u003e\n\u003ctd\u003e100 columns\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e100 x E. coli topoisomerase IV (500 nM)\u003c\/td\u003e\n\u003ctd\u003eNot included\u003c\/td\u003e\n\u003ctd\u003e12 µl\u003c\/td\u003e\n\u003ctd\u003e55 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cp\u003eThe assay buffer is optimized for bacterial topoisomerase IV. Final reaction conditions: 20 mM Tris-HCl pH 8, 35 mM NH4OAc, 4.6% glycerol, 1 mM DTT, 0.005% Brij35, 8 mM MgCl2, 2 µg\/ml concatenated DNA, 0.2 mM ATP and 5 nM topoisomerase IV.\u003c\/p\u003e\n\u003ch2\u003eAssay Principle\u003c\/h2\u003e\n\u003cp\u003eAfter the decatenation reaction, a quick spin-column step separates the products: concatenated DNA stays on the column while decatenated DNA passes through. The eluted DNA is mixed with a fluorescence dye and read at Em 535 nm (Ex 485 nm), so the signal rises with decatenation activity.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Target\u003c\/th\u003e\n\u003ctd\u003eTopo IV DNA decatenation activity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget Type\u003c\/th\u003e\n\u003ctd\u003eDNA Topoisomerase IV\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOrganism\u003c\/th\u003e\n\u003ctd\u003eE. coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Type\u003c\/th\u003e\n\u003ctd\u003eEnzyme Activity Assay\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Method\u003c\/th\u003e\n\u003ctd\u003eFluorescence\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Wavelength\u003c\/th\u003e\n\u003ctd\u003eEx 485 nm \/ Em 535 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eInstrument Required\u003c\/th\u003e\n\u003ctd\u003eFluorescence reader + centrifuge\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Time\u003c\/th\u003e\n\u003ctd\u003eAbout 65 min (60 min reaction + 4.5 min spin-column steps)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSizes\u003c\/th\u003e\n\u003ctd\u003e20 assays, 100 assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm storage conditions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eShipped frozen.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eProtocol\u003c\/h2\u003e\n\u003cp\u003eThe manufacturer's protocol describes the procedure and reaction volumes.\u003c\/p\u003e\n\u003cul\u003e\u003cli\u003eDNA Topoisomerase IV Assay (PDF, see Documents): Covers catalog # DDC020K, DDC020KE, DDC100KE.\u003c\/li\u003e\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eFor Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. The DNA Topoisomerase DNA Decatenation Assay Kit is shipped frozen. Follow standard laboratory safety practices when handling the reagents. Please contact us to confirm storage conditions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ProFoldin","offers":[{"title":"Kit (enzyme not included) \/ 20 assays","offer_id":67794879054189,"sku":"DDC020K","price":339.0,"currency_code":"USD","in_stock":true},{"title":"Kit Plus (E. coli Topo IV included) \/ 20 assays","offer_id":67794882560365,"sku":"DDC020KE","price":409.0,"currency_code":"USD","in_stock":true},{"title":"Kit Plus (E. coli Topo IV included) \/ 100 assays","offer_id":67794882593133,"sku":"DDC100KE","price":1208.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/DNA_dec-640.jpg?v=1792791969","url":"https:\/\/www.ebiohippo.com\/products\/dna-topoisomerase-dna-decatenation-assay-kit-bht16300083","provider":"BioHippo","version":"1.0","type":"link"}