{"product_id":"dnajb6-antibody-dnaj-homolog-subfamily-b-member-6-bha17135279","title":"DNAJB6 Antibody \/ DnaJ homolog subfamily B member 6","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eDNAJB6 Antibody \/ DnaJ homolog subfamily B member 6 is a anti-DNAJB6 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasmic, Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e DNAJB6\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe DNAJB6 antibody targets DnaJ homolog subfamily B member 6, a molecular chaperone encoded by the DNAJB6 gene. This protein belongs to the Hsp40 (DnaJ) family, which functions as a co-chaperone of Hsp70, facilitating protein folding, quality control, and aggregation prevention. DnaJ homolog subfamily B member 6 plays a key role in maintaining proteostasis and protecting cells from misfolded protein stress. The DNAJB6 antibody enables specific detection of this versatile chaperone, making it an essential reagent for studying protein homeostasis, neurodegeneration, and myopathies.\u003cbr\u003e\u003cbr\u003eDnaJ homolog subfamily B member 6 exists in two major isoforms produced by alternative splicing: a long nuclear\/cytoplasmic form (DNAJB6a) and a shorter cytoplasmic form (DNAJB6b). Both isoforms cooperate with Hsp70 to stabilize unfolded or aggregation-prone proteins, particularly under stress conditions such as heat shock or oxidative damage. The DNAJB6 antibody detects these isoforms, allowing analysis of expression patterns and subcellular localization in response to proteotoxic stress.\u003cbr\u003e\u003cbr\u003eMutations in DNAJB6 cause limb-girdle muscular dystrophy type D1 (LGMDD1), a progressive neuromuscular disorder characterized by protein aggregation and muscle weakness. The DNAJB6 antibody is crucial for studying the molecular basis of this disease, enabling quantification of mutant and wild-type protein levels and their aggregation propensity. DnaJ homolog subfamily B member 6 has also been shown to suppress polyglutamine aggregation in Huntington's disease models, highlighting its broader neuroprotective role. The DNAJB6 antibody supports investigations into these mechanisms by providing reliable detection in neuronal and muscle tissues.\u003cbr\u003e\u003cbr\u003eBeyond its function in proteostasis, DnaJ homolog subfamily B member 6 participates in transcriptional regulation and signal transduction. It interacts with components of the Wnt\/beta-catenin pathway, influencing gene expression and cellular differentiation. The DNAJB6 antibody allows exploration of these noncanonical functions, revealing how chaperones integrate protein quality control with developmental signaling. Elevated expression of DNAJB6 has been associated with enhanced stress resistance and reduced metastasis in some cancer cells, further emphasizing its physiological relevance.\u003cbr\u003e\u003cbr\u003eThe DNAJB6 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry. It provides clear cytoplasmic and nuclear localization depending on isoform distribution.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=DNAJB6 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DNAJB6 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=DNAJB6 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/DNAJB6 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=DNAJB6+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047288070509,"sku":"FY12376","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_92c5ed9c-68ab-49ce-908c-d34f9e77b749.jpg?v=1782237005","url":"https:\/\/www.ebiohippo.com\/products\/dnajb6-antibody-dnaj-homolog-subfamily-b-member-6-bha17135279","provider":"BioHippo","version":"1.0","type":"link"}