{"product_id":"dnase-i-bhp15200311","title":"Deoxyribonuclease I (DNase I)","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}.bhp-desc .bhp-comp-table th{width:auto}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eNucleases \u0026amp; Proteases\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eDeoxyribonuclease I (DNase I) is a recombinant DNase I protein expressed in E. coli, without RNase. Hydrolysis of single- or double-stranded DNA leaves a phosphate group at the 5' end and a hydroxyl group at the 3' end. DNase I activity is dependent on calcium ions and can be activated by magnesium ions or divalent manganese ions. In the presence of magnesium ions, DNase I can randomly cleave any site of double-stranded DNA; in the presence of divalent manganese ions, DNase I can cleave DNA double-strands at the same site to form blunt ends or short sticky ends with 1–2 nucleotide overhangs.\u003c\/p\u003e\n\u003cp\u003eThe enzyme is supplied as a single reagent rather than a kit, in packs of 1000 U (500 µL) and 2000 U (1 mL). Documented uses are removal of DNA contamination from RNA samples, elimination of DNA template after transcription, nick translation, DNase I footprinting for protein–DNA interaction studies, generation of random DNA fragment libraries and use as a positive control in apoptosis TUNEL assays.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003eSingle reagent, recombinant DNase I solution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSource\u003c\/th\u003e\n\u003ctd\u003eRecombinant (E. coli)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eRNase content\u003c\/th\u003e\n\u003ctd\u003eWithout RNase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSubstrate\u003c\/th\u003e\n\u003ctd\u003eSingle- or double-stranded DNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCofactor requirement\u003c\/th\u003e\n\u003ctd\u003eActivity is dependent on calcium ions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eActivation by Mg²⁺\u003c\/th\u003e\n\u003ctd\u003eRandom cleavage at any site of double-stranded DNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eActivation by Mn²⁺\u003c\/th\u003e\n\u003ctd\u003eCleavage of both strands at the same site, giving blunt ends or 1–2 nucleotide overhangs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProduct ends\u003c\/th\u003e\n\u003ctd\u003e5' phosphate group and 3' hydroxyl group\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRemoval of DNA contamination from RNA samples and of DNA template after transcription\u003c\/li\u003e\n\u003cli\u003eNick translation\u003c\/li\u003e\n\u003cli\u003eDNase I footprinting for protein–DNA interaction studies\u003c\/li\u003e\n\u003cli\u003eGeneration of random DNA fragment libraries\u003c\/li\u003e\n\u003cli\u003ePositive control in apoptosis TUNEL assays\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKey Features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eNo cumbersome phenol\/chloroform extraction or inactivation is required after digestion.\u003c\/li\u003e\n\u003cli\u003eThe digested sample can be used directly in a reverse transcription reaction, which is fast and easy to use.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eStored at room temperature with a shelf life of one year.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eFor research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.\u003c\/p\u003e\u003c\/div\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eGrade\u003c\/strong\u003eResearch Use Only\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eManufacturer\u003c\/strong\u003eELK Biotechnology\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eCatalog No.\u003c\/strong\u003eEQ025\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eBioHippo SKU\u003c\/strong\u003eBHP15200311\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"1000 U (500 uL)","offer_id":53965434290541,"sku":"EQ025-1000U","price":0.0,"currency_code":"USD","in_stock":true},{"title":"2000 U (1 mL)","offer_id":53965434487149,"sku":"EQ025-2000U","price":0.0,"currency_code":"USD","in_stock":true},{"title":"2000 U x 10","offer_id":53965434519917,"sku":"EQ025-2000UX10","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EQ025-a-1.jpg?v=1789416776","url":"https:\/\/www.ebiohippo.com\/products\/dnase-i-bhp15200311","provider":"BioHippo","version":"1.0","type":"link"}