{"product_id":"dnasecure-plant-kit-bht15200011","title":"DNAsecure Plant Kit","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}.bhp-desc .bhp-comp-table th{width:auto}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eDNA Extraction Kits\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThis kit uses a unique buffer system and is especially suitable for extracting genomic DNA from fresh plant materials. It is safe and convenient to use. It can remove impurities, proteins and other organic compounds in cells to the greatest extent. This product does not contain harmful chemical components such as phenol, chloroform and so on. It is safer for the operator. The kit yields high-molecular-weight genomic DNA of high purity. The DNA recovered by this kit can be used in various routine operations, including enzyme digestion, PCR, library construction, Southern hybridization and so on.\u003c\/p\u003e\n\u003cp\u003ePlant tissue of 100 mg is ground under liquid nitrogen and lysed in 600 µL of Buffer PGE with 6 µL of RNase A at 60°C for 20 min. The cleared lysate is mixed with 0.3 volumes of absolute ethanol and loaded onto the adsorption column, washed with 500 µL of Solution RP and twice with 600 µL of Wash Buffer, air-dried for 5–10 min and eluted with 30–50 µL of Elution Buffer. Purified DNA has an OD260\/OD280 of 1.7–1.9.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003eSpin column\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample type\u003c\/th\u003e\n\u003ctd\u003eFresh plant material\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample input\u003c\/th\u003e\n\u003ctd\u003e100 mg fresh tissue or 20 mg dry weight\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eYield\u003c\/th\u003e\n\u003ctd\u003e3–30 µg genomic DNA from 100 mg plant tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003eOD260\/OD280 should be 1.7–1.9\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProcessing time\u003c\/th\u003e\n\u003ctd\u003eWithin 1 h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eChemistry\u003c\/th\u003e\n\u003ctd\u003ePhenol\/chloroform-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eElution volume\u003c\/th\u003e\n\u003ctd\u003e30–50 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table bhp-comp-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003e50 T\u003c\/th\u003e\n\u003cth\u003e200 T\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer PGE\u003c\/td\u003e\n\u003ctd\u003e35 mL\u003c\/td\u003e\n\u003ctd\u003e140 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer\u003c\/td\u003e\n\u003ctd\u003e60 mL\u003c\/td\u003e\n\u003ctd\u003e240 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSolution RP\u003c\/td\u003e\n\u003ctd\u003e30 mL\u003c\/td\u003e\n\u003ctd\u003e120 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eElution Buffer\u003c\/td\u003e\n\u003ctd\u003e2.5 mL\u003c\/td\u003e\n\u003ctd\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRNase A\u003c\/td\u003e\n\u003ctd\u003e315 µL\u003c\/td\u003e\n\u003ctd\u003e1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdsorption column\u003c\/td\u003e\n\u003ctd\u003e50 set\u003c\/td\u003e\n\u003ctd\u003e200 set\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eGenomic DNA extraction from fresh plant tissue\u003c\/li\u003e\n\u003cli\u003eEnzyme digestion and PCR\u003c\/li\u003e\n\u003cli\u003eLibrary construction\u003c\/li\u003e\n\u003cli\u003eSouthern hybridization\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKey Features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eSimple and fast: High purity genomic DNA can be obtained within 1 h.\u003c\/li\u003e\n\u003cli\u003eSafe and low toxicity: No need to use phenol\/chloroform extraction, safe operation.\u003c\/li\u003e\n\u003cli\u003eHigh purity and good quality: 3–30 µg of genomic DNA can be extracted from 100 mg of plant tissue, and the value of OD260\/OD280 is in the range of 1.7–1.9.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eBuffer PGE, Wash Buffer, Solution RP, Elution Buffer and the adsorption columns are stored at room temperature for 1 year. RNase A is stored at -20°C for 1 year.\u003c\/p\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles.\u003c\/li\u003e\n\u003cli\u003ePre-dissolve precipitated Buffer PGE at 56°C before use.\u003c\/li\u003e\n\u003cli\u003eUse fresh plant tissue.\u003c\/li\u003e\n\u003cli\u003eAdd absolute ethanol to the Wash Buffer before first use, as instructed in the manual.\u003c\/li\u003e\n\u003cli\u003eAir-dry the column for 5–10 min at room temperature before elution.\u003c\/li\u003e\n\u003cli\u003eThe dissolved volume affects both yield and final concentration.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eFor research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.\u003c\/p\u003e\u003c\/div\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eGrade\u003c\/strong\u003eResearch Use Only\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eManufacturer\u003c\/strong\u003eELK Biotechnology\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eCatalog No.\u003c\/strong\u003eEP010\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eBioHippo SKU\u003c\/strong\u003eBHT15200011\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"50 T","offer_id":53965512081773,"sku":"EP010-50T","price":130.0,"currency_code":"USD","in_stock":true},{"title":"200 T","offer_id":53965516964205,"sku":"EP010-200T","price":364.0,"currency_code":"USD","in_stock":true},{"title":"50 T x 10","offer_id":53965516996973,"sku":"EP010-50TX10","price":0.0,"currency_code":"USD","in_stock":true},{"title":"200 T x 5","offer_id":53965517029741,"sku":"EP010-200TX5","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EP010-a-1.jpg?v=1789419190","url":"https:\/\/www.ebiohippo.com\/products\/dnasecure-plant-kit-bht15200011","provider":"BioHippo","version":"1.0","type":"link"}