{"product_id":"dock8-antibody-dedicator-of-cytokinesis-protein-8-bha17135322","title":"DOCK8 Antibody \/ Dedicator of cytokinesis protein 8","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eDOCK8 Antibody \/ Dedicator of cytokinesis protein 8 is a anti-DOCK8 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, cell membrane.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e DOCK8\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe DOCK8 antibody targets Dedicator of cytokinesis protein 8, a large guanine nucleotide exchange factor encoded by the DOCK8 gene. Dedicator of cytokinesis protein 8 regulates cytoskeletal dynamics by activating small GTPases of the Rho family, particularly CDC42, thereby controlling immune cell morphology, migration, and synapse formation. The DOCK8 antibody provides an essential tool for studying immune signaling, actin remodeling, and primary immunodeficiency syndromes associated with DOCK8 dysfunction.\u003cbr\u003e\u003cbr\u003eDedicator of cytokinesis protein 8 contains a DOCK homology region (DHR) that mediates binding to CDC42 and phosphatidylinositol lipids. This structural configuration facilitates the localized activation of actin polymerization at the immunological synapse. The DOCK8 antibody enables visualization of this process, revealing how immune cells such as T cells, B cells, and natural killer cells use DOCK8 to maintain polarity and movement. Loss of function in DOCK8 disrupts actin organization, impairing immune synapse stability and antigen-specific responses.\u003cbr\u003e\u003cbr\u003eMutations in the DOCK8 gene cause autosomal recessive hyper-immunoglobulin E syndrome (HIES), characterized by recurrent viral infections, elevated IgE levels, and defective T and B cell function. The DOCK8 antibody supports studies into this condition by allowing detection of protein expression in immune cell subsets from affected patients. Functional loss of Dedicator of cytokinesis protein 8 leads to increased susceptibility to viral skin infections and defective immune surveillance, highlighting its critical role in immune defense.\u003cbr\u003e\u003cbr\u003eBeyond immunodeficiency, Dedicator of cytokinesis protein 8 has been implicated in cancer biology, where its dysregulation can influence metastasis and tumor cell migration. The DOCK8 antibody supports investigations into these broader roles by enabling quantification of expression in cancer cell lines and tissues. Through regulation of actin assembly and cell adhesion, DOCK8 contributes to both immune and non-immune cell motility control.\u003cbr\u003e\u003cbr\u003eThe DOCK8 antibody performs effectively in western blotting, immunofluorescence, and flow cytometry, showing strong cytoplasmic localization consistent with its role in actin remodeling.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=DOCK8 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DOCK8 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=DOCK8 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/DOCK8 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=DOCK8+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047287841133,"sku":"FY12419","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_094af8a3-f027-4477-ade1-3c96dda883b8.jpg?v=1782237002","url":"https:\/\/www.ebiohippo.com\/products\/dock8-antibody-dedicator-of-cytokinesis-protein-8-bha17135322","provider":"BioHippo","version":"1.0","type":"link"}