{"product_id":"dog-fgf23-fibroblast-growth-factor-23-elisa-kit-bhe15212802","title":"Dog FGF23(Fibroblast Growth Factor 23) ELISA Kit","description":"\u003ch3\u003eScientific background\u003c\/h3\u003e\u003cp\u003e\u003cstrong\u003eFGF23 (Fibroblast Growth Factor 23)\u003c\/strong\u003e is a growth factor–related marker involved in cellular proliferation, differentiation, or tissue remodeling processes.\u003c\/p\u003e\u003cp\u003eGrowth factor concentrations can reflect changes in tissue repair, fibrosis, angiogenesis, or tumor microenvironment signaling depending on the specific target and model.\u003c\/p\u003e\u003cp\u003eBecause many growth factors act locally and are regulated post-transcriptionally, protein quantification can add clarity beyond mRNA-only measurements.\u003c\/p\u003e\u003ch3\u003eWhy it matters\u003c\/h3\u003e\u003cul\u003e\n\u003cli\u003eQuantify \u003cstrong\u003eFGF23 (Fibroblast Growth Factor 23)\u003c\/strong\u003e to compare biological changes across conditions, doses, or time points.\u003c\/li\u003e\n\u003cli\u003eGenerate concentration data from a standard curve to support biomarker and mechanistic studies.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch3\u003eHow the ELISA works\u003c\/h3\u003e\u003cp\u003eDesigned for \u003cstrong\u003eDog\u003c\/strong\u003e samples, this kit uses a \u003cstrong\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog FGF23. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog FGF23. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog FGF23, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog FGF23 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/strong\u003e. After binding and washing, signal is converted to concentration using a standard curve.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSample types\u003c\/strong\u003e: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection range\u003c\/strong\u003e: 15.63-1000 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSensitivity\/LoD\u003c\/strong\u003e: 5.7 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay time\u003c\/strong\u003e: 3.5h\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"ELK Biotechnology","offers":[{"title":"96 T","offer_id":52966454886765,"sku":"ELK11216-96T","price":595.4,"currency_code":"USD","in_stock":true},{"title":"48 T","offer_id":52966454919533,"sku":"ELK11216-48T","price":416.0,"currency_code":"USD","in_stock":true},{"title":"96 T X 5","offer_id":52966454952301,"sku":"ELK11216-96TX5","price":2531.1,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1h1qbq4v21p4717oo1b_d9a2c56e-419b-4ac3-a892-a0ea2906fe69.jpg?v=1769437160","url":"https:\/\/www.ebiohippo.com\/products\/dog-fgf23-fibroblast-growth-factor-23-elisa-kit-bhe15212802","provider":"BioHippo","version":"1.0","type":"link"}