{"product_id":"donkey-anti-mouse-igg-ap-bha19900793","title":"Donkey Anti-mouse IgG, AP","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eDonkey Anti-mouse IgG, AP is a research polyclonal targeting Mouse IgG, supplied as a AP format for FC \/ IF workflows. It supports measurement of Mouse target expression in common experimental systems.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eClone:\u003c\/strong\u003e Polyclonal — consistent clone identity can support panel reproducibility and cross-study comparisons.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype:\u003c\/strong\u003e Polyclone Ig — informs selection of matched controls and secondary reagents when relevant.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConjugate:\u003c\/strong\u003e AP — enables direct detection in fluorescence-based assays.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReactivity:\u003c\/strong\u003e Mouse — interpret staining in the context of species-specific sequence and expression differences.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eKey specifications such as clone identity, isotype, and fluorophore conjugation help researchers align panel design, control selection, and instrument configuration with the biological question and sample type.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eThis antibody reacts with the heavy chains of mouse IgG and with the light (kappa and lambda) chains common to most mouse immunoglobulins. The minimal cross-reaction with other species was ensured by pre-adsorption by agarose beads coupled with IgGs from Human, Goat, Rabbit, Bovine, Chicken, Guinea Pig, Syrian Hamster, Horse and Sheep Serum Proteins.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eHigh-parameter immunophenotyping: combining Mouse IgG with complementary lineage and activation markers to resolve complex cell states.\u003c\/li\u003e\n\u003cli\u003ePanel standardization and data comparability: increasing emphasis on consistent reagents, compensation-aware fluorophore choices, and shared gating strategies.\u003c\/li\u003e\n\u003cli\u003eIntegration with single-cell multi-omics: pairing surface marker profiling with transcriptomic or proteomic readouts to connect phenotype to function.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eFlow cytometry: quantify Mouse IgG-positive populations and compare expression distributions across conditions or time points.\u003c\/li\u003e\n\u003cli\u003eCell sorting: enrich Mouse IgG-defined subsets for downstream RNA\/protein assays or functional readouts.\u003c\/li\u003e\n\u003cli\u003eImmunofluorescence: visualize cellular distribution of Mouse IgG and assess co-localization with other markers.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eChanges in measured signal are typically interpreted in the context of cell subset frequency, activation\/differentiation state, and sample processing effects rather than as a standalone readout.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eFluorophore selection: consider brightness, spectral overlap, and instrument configuration; compensation and spillover can affect apparent population boundaries.\u003c\/li\u003e\n\u003cli\u003eBiology-driven confounders: activation state, differentiation, and isoform\/PTM variation can shift epitope accessibility and apparent expression.\u003c\/li\u003e\n\u003cli\u003eControl concepts: include matched isotype and fluorescence-minus-one (FMO) controls where appropriate, and interpret results alongside biological positive\/negative reference samples.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eFor antibody-based assays, monoclonal versus polyclonal format can influence epitope recognition breadth and signal consistency. Conjugated antibodies support direct detection and can simplify multicolor panel design when paired with appropriate controls and instrument settings.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt Knowledgebase — UniProt — https:\/\/www.uniprot.org\/ - NCBI Gene — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/ - HGNC gene nomenclature — HUGO Gene Nomenclature Committee — https:\/\/www.genenames.org\/ - Flow cytometry basics — NIH\/NCI (overview resources) — https:\/\/www.cancer.gov\/research\/resources - High-dimensional cytometry overview — Nature Methods (journal) — https:\/\/www.nature.com\/nmeth\/ --\u003e","brand":"Caprico","offers":[{"title":"0.5 mg","offer_id":53072776528237,"sku":"210041","price":208.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Picture5.png?v=1772634910","url":"https:\/\/www.ebiohippo.com\/products\/donkey-anti-mouse-igg-ap-bha19900793","provider":"BioHippo","version":"1.0","type":"link"}