{"product_id":"dot1l-antibody-kmt4-bha17136366","title":"DOT1L Antibody \/ KMT4","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eDOT1L Antibody \/ KMT4 is a anti-DOT1L Rabbit antibody Recombinant Rabbit Monoclonal clone CBF-4 supplied in Purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Human, Mouse.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e DOT1L\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone CBF-4, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eDOT1L antibody is a widely used reagent for studying epigenetic control, chromatin remodeling, and transcriptional regulation. The encoded protein, KMT4 (also known as disruptor of telomeric silencing 1 like), is a histone methyltransferase that specifically methylates histone H3 on lysine 79. This unique modification, located within the globular domain of histone H3, is strongly linked to transcriptional activation, DNA repair, and maintenance of genomic stability. Unlike most lysine methyltransferases that contain a SET domain, DOT1L is structurally distinct, highlighting its specialized role in epigenetic regulation.\u003cbr\u003e\u003cbr\u003eKMT4 functions by altering chromatin accessibility and creating binding sites for regulatory proteins that control transcription and DNA damage response. During development, DOT1L plays critical roles in embryogenesis, hematopoiesis, and differentiation. Loss of DOT1L disrupts stem cell renewal and lineage commitment, demonstrating its essential role in developmental biology. Its activity is also important for maintaining telomeric silencing and ensuring proper chromosomal function.\u003cbr\u003e\u003cbr\u003eAberrant DOT1L activity has been strongly implicated in cancer. In mixed lineage leukemia (MLL) rearranged leukemias, DOT1L is aberrantly recruited to oncogenic transcriptional complexes, leading to inappropriate H3K79 methylation and activation of leukemia promoting genes. This discovery has made DOT1L a therapeutic target, and small molecule inhibitors of DOT1L are in clinical trials for treatment of MLL associated leukemias. Beyond leukemia, dysregulated DOT1L activity has been observed in solid tumors, cardiovascular disease, and viral infection, underscoring its wide biological significance.\u003cbr\u003e\u003cbr\u003eAt the molecular level, DOT1L associates with transcriptional elongation complexes and other chromatin modifiers, linking histone modification with gene expression control. Structural studies show that DOT1L interacts directly with the nucleosome core, uniquely positioning it to catalyze methylation of H3K79. Through these mechanisms, DOT1L serves as an integrator of epigenetic signaling and transcriptional activity, shaping the cellular transcriptome in response to developmental and environmental signals.\u003cbr\u003e\u003cbr\u003eThe DOT1L antibody is commonly used in western blotting, immunohistochemistry, immunofluorescence, and flow cytometry to detect expression levels, nuclear localization, and disease associated changes. These applications are valuable for exploring transcriptional regulation, epigenetic therapy, and cancer biology. For researchers studying chromatin structure, RNA polymerase II elongation, or targeted therapeutics, the DOT1L antibody provides a reliable and specific detection tool.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=DOT1L - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DOT1L - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=DOT1L - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/DOT1L - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=DOT1L+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047327097197,"sku":"RQ8948","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_473cff9e-70a0-4054-a18e-392bb9cc9c33.jpg?v=1772019450","url":"https:\/\/www.ebiohippo.com\/products\/dot1l-antibody-kmt4-bha17136366","provider":"BioHippo","version":"1.0","type":"link"}