{"product_id":"dr3-nf-kappa-b-reporter-lentivirus-bhv19400260","title":"DR3\/NFκB Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eDR3 (death receptor 3) is a member of the tumor necrosis factor receptor superfamily expressed predominantly on T lymphocytes and other immune cells. Its ligand, TL1A, engages DR3 to activate downstream signaling that can promote both T cell costimulation and, depending on context, pro-apoptotic responses. A major branch of DR3 signaling activates the NF-κB transcription factor, driving expression of genes involved in inflammation, immune cell activation, and survival. The TL1A-DR3 axis modulates T cell responses and is implicated in inflammatory and autoimmune diseases, making it an important target in immunology and immunotherapy research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe DR3\/NFκB Reporter Lentivirus is an immunotherapy reporter system for studying DR3 receptor-mediated signaling. The construct constitutively expresses the DR3 receptor, which upon engagement by its ligand TL1A activates the NF-κB pathway, driving a dual reporter of secreted Gaussia luciferase and a fluorescent protein. This provides a quantitative readout of pro-apoptotic and inflammatory signaling and supports studies of receptor-engagement inhibition by blocking compounds or antibodies. Sequential transduction generates a stable reporter cell line, and secreted Gaussia luciferase enables non-destructive kinetic sampling from conditioned media. Supplied as high-titer, VSV-G-pseudotyped third-generation lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation, optimized for difficult-to-transfect cells, including primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis 2-vial immunotherapy reporter system consists of a \u003cstrong\u003eVial 1 Receptor Lentivirus\u003c\/strong\u003e encoding human DR3 under a constitutive promoter with antibiotic selection, and a \u003cstrong\u003eVial 2 Reporter Lentivirus\u003c\/strong\u003e encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (GFP, GFP-P2A-GLuc, GLuc, GLuc-P2A-GFP, GLuc-P2A-RFP, RFP, RFP-P2A-GLuc). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.\u003c\/p\u003e\n\u003cp\u003eSecreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GLuc-P2A-GFP \/ Blasticidin \/ 2x10^6","offer_id":53251619357037,"sku":"TRV-0011-6S","price":895.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/dr3-nf-kappa-b-reporter-lentivirus-bhv19400260","provider":"BioHippo","version":"1.0","type":"link"}