{"product_id":"dtnbp1-antibody-dysbindin-bha17135732","title":"DTNBP1 Antibody \/ Dysbindin","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eDTNBP1 Antibody \/ Dysbindin is a anti-DTNBP1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e DTNBP1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eDTNBP1 antibody detects Dystrobrevin-binding protein 1 (commonly known as Dysbindin), a cytoplasmic protein involved in vesicle trafficking, synaptic function, and organelle biogenesis. Encoded by the DTNBP1 gene on chromosome 6p22.3, this protein is a core component of the biogenesis of lysosome-related organelles complex 1 (BLOC-1). Through its scaffolding and trafficking functions, Dysbindin regulates endosomal transport, melanosome formation, and neurotransmitter release, particularly within neuronal and pigment cells.\u003cbr\u003e\u003cbr\u003eDysbindin interacts with other BLOC-1 subunits, including SNAPIN, MUTED, and BLOC1S proteins, coordinating actin-based vesicular transport. In neurons, it contributes to synaptic vesicle recycling and glutamatergic signaling, influencing synaptic plasticity and cognitive processes. Dysbindin localizes to endosomes and synaptic vesicles, reflecting its role in membrane trafficking and receptor recycling.\u003cbr\u003e\u003cbr\u003eThe DTNBP1 antibody is widely used in neuroscience, cell biology, and genetic disease research to examine endosomal transport, synaptic vesicle dynamics, and intracellular trafficking. Western blot analysis identifies a 40 kilodalton band corresponding to Dysbindin, while immunofluorescence shows punctate cytoplasmic staining consistent with endosomal localization. This antibody supports investigations into molecular mechanisms of vesicle regulation and neurotransmission.\u003cbr\u003e\u003cbr\u003eLoss-of-function mutations or reduced expression of DTNBP1 have been associated with Hermansky-Pudlak syndrome type 7 and susceptibility to schizophrenia, linking Dysbindin to synaptic homeostasis and neurodevelopment. Dysbindin also influences muscle membrane stability by binding to dystrobrevins. The DTNBP1 antibody provides a dependable reagent for exploring vesicle trafficking, neurological function, and genetic disorders.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=DTNBP1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DTNBP1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=DTNBP1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/DTNBP1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=DTNBP1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047303176557,"sku":"FY12830","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_cad3e025-a350-48d1-aeb8-1f6cdd1adfc3.jpg?v=1782237052","url":"https:\/\/www.ebiohippo.com\/products\/dtnbp1-antibody-dysbindin-bha17135732","provider":"BioHippo","version":"1.0","type":"link"}