{"product_id":"dut-antibody-deoxyuridine-triphosphatase-bha17135588","title":"DUT Antibody \/ Deoxyuridine triphosphatase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eDUT Antibody \/ Deoxyuridine triphosphatase is a anti-DUT Rabbit antibody Recombinant Rabbit Monoclonal clone 31D50 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e DUT\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 31D50, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eDUT antibody detects deoxyuridine triphosphatase, an enzyme encoded by the DUT gene. This enzyme catalyzes the hydrolysis of dUTP to dUMP and pyrophosphate, thereby preventing the incorporation of uracil into DNA. This reaction maintains the balance of nucleotide pools and supports DNA replication fidelity. DUT is expressed in both nuclear and mitochondrial isoforms, generated through alternative splicing, enabling the enzyme to function across different subcellular compartments where nucleotide metabolism is critical.\u003cbr\u003e\u003cbr\u003eDUT antibody is widely applied in DNA metabolism research, cancer biology, and virology. In DNA replication, DUT regulates the concentration of dUTP to minimize misincorporation of uracil into DNA. Failure to control dUTP levels leads to DNA repair cycles, strand breaks, and genomic instability. By detecting DUT, researchers can examine the mechanisms that safeguard DNA integrity and how these processes become dysregulated in disease.\u003cbr\u003e\u003cbr\u003e Western blotting with DUT antibody detects the nuclear and mitochondrial isoforms of the protein in proliferative tissues. Immunohistochemistry maps DUT expression in tissues with high replication rates such as bone marrow and intestinal crypts. Immunofluorescence highlights the nuclear and mitochondrial distribution of DUT within single cells. ELISA allows quantification of enzyme expression in experimental and clinical samples. These approaches make DUT antibody a versatile reagent for investigating DNA metabolism and genomic stability.\u003cbr\u003e\u003cbr\u003eDUT is especially important in rapidly dividing cells and is upregulated during S phase of the cell cycle. This makes DUT an attractive target in oncology, since cancer cells often rely on high levels of DUT to manage imbalanced nucleotide pools. Inhibition of DUT activity has been explored as a strategy to increase DNA damage selectively in tumor cells. By applying DUT antibody, scientists can explore how altered nucleotide metabolism contributes to tumorigenesis and identify therapeutic opportunities.\u003cbr\u003e\u003cbr\u003eBeyond cancer, DUT plays roles in virology. Several viruses, including HIV, exploit host nucleotide metabolism, and DUT expression influences viral replication. DUT activity may affect the effectiveness of nucleoside analog drugs by altering nucleotide balance. These insights make DUT antibody a useful tool for studying virus-host interactions and antiviral drug responses.\u003cbr\u003e\u003cbr\u003eDUT also contributes to developmental biology. The enzyme supports DNA synthesis during embryogenesis and tissue regeneration. Genetic mutations or dysregulation of DUT are linked to cellular stress responses and mitochondrial dysfunction. Studying DUT with antibody based approaches provides insights into both normal development and pathological processes.\u003cbr\u003e\u003cbr\u003eDUT antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=DUT - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=DUT - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=DUT - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/DUT - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=DUT+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047297605997,"sku":"FY12685","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_2b6b567c-264a-4e57-8d86-e430ba7f3ece.jpg?v=1772019329","url":"https:\/\/www.ebiohippo.com\/products\/dut-antibody-deoxyuridine-triphosphatase-bha17135588","provider":"BioHippo","version":"1.0","type":"link"}