{"product_id":"e-coli-dna-gyrase-bhp16300029","title":"E. coli DNA Gyrase","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ol,.bhp-desc ul{margin:0 0 12px 20px}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eEnzyme\u003c\/span\u003e\u003cp\u003eBacterial DNA topoisomerase II (gyrase) is a type II topoisomerase that converts relaxed circular DNA into negatively supercoiled DNA. Type II topoisomerases cut and rejoin both DNA strands to change DNA topology.\u003c\/p\u003e\n\u003cp\u003eSome inhibitors, such as fluoroquinolones, block the rejoining step; the enzyme then stays covalently linked to cut DNA in a cleavage complex. Ciprofloxacin and novobiocin both inhibit gyrase, but only ciprofloxacin produces cleavage complexes.\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctr\u003e\n\u003cth\u003e100 assays – TOP2-100EC\u003c\/th\u003e\n\u003ctd\u003e40 µl of 2 µM wild-type E. coli gyrase stock solution.\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/table\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eProduct Type\u003c\/th\u003e\n\u003ctd\u003eDNA Topoisomerases\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOrganism\u003c\/th\u003e\n\u003ctd\u003eE. coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConcentration\u003c\/th\u003e\n\u003ctd\u003e2 µM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSizes\u003c\/th\u003e\n\u003ctd\u003e100 assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog #\u003c\/th\u003e\n\u003ctd\u003eTOP2-100EC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm storage conditions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eShipped frozen.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eUse With\u003c\/h2\u003e\n\u003cp\u003eThis enzyme is used in the following ProFoldin assay kits:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca href=\"\/products\/96-well-e-coli-gyrase-dna-cleavage-assay-kit-plus-bht16300063\"\u003e96-Well E. coli Gyrase DNA Cleavage Assay Kit Plus\u003c\/a\u003e (BHT16300063) — complete kit: already includes this enzyme\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"\/products\/96-well-e-coli-gyrase-dna-decatenation-assay-kit-plus-bht16300065\"\u003e96-Well E. coli Gyrase DNA Decatenation Assay Kit Plus\u003c\/a\u003e (BHT16300065) — complete kit: already includes this enzyme\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"\/products\/e-coli-dna-topoisomerase-ii-gyrase-assay-kit-bht16300067\"\u003eE. coli DNA Topoisomerase II (Gyrase) Assay Kit\u003c\/a\u003e (BHT16300067) — DSA100KE already includes this enzyme; DSA100K is enzyme-free and needs this product\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"\/products\/e-coli-gyrase-atpase-assay-kit-plus-bht16300068\"\u003eE. coli Gyrase ATPase Assay Kit Plus\u003c\/a\u003e (BHT16300068) — complete kit: already includes this enzyme\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"\/products\/gel-based-e-coli-dna-topoisomerase-ii-gyrase-assay-kit-plus-bht16300069\"\u003eGel-Based E. coli DNA Topoisomerase II (Gyrase) Assay Kit Plus\u003c\/a\u003e (BHT16300069) — complete kit: already includes this enzyme\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtocol\u003c\/h2\u003e\n\u003cp\u003eNo separate datasheet is available for this enzyme. The ProFoldin protocols “Topoisomerase DNA Cleavage Assays”, “E. coli DNA Topoisomerase II (Gyrase) Assay”, “DNA Topoisomerase ATPase Assays” and “Gel-Based E. coli Gyrase DNA Supercoiling Assay” (linked under Documents) show how the enzyme is used in the matching assay kits.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eFor Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. This enzyme is shipped frozen. Follow standard laboratory safety practices.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ProFoldin","offers":[{"title":"100 assays","offer_id":67815135248749,"sku":"TOP2-100EC","price":570.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/e-coli-dna-gyrase-bhp16300029","provider":"BioHippo","version":"1.0","type":"link"}