{"product_id":"e-coli-dna-topoisomerase-i-bhp16300028","title":"E. coli DNA Topoisomerase I","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ol,.bhp-desc ul{margin:0 0 12px 20px}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eEnzyme\u003c\/span\u003e\u003cp\u003eDNA relaxation is the change of negatively supercoiled circular DNA into relaxed DNA, carried out by DNA topoisomerases such as bacterial topoisomerase I. Topoisomerase I can also decatenate DNA circles when at least one of them is nicked.\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctr\u003e\n\u003cth\u003e100 assays – TOP1-100EC\u003c\/th\u003e\n\u003ctd\u003e42 µl of 4 µM E. coli topoisomerase I.\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/table\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eProduct Type\u003c\/th\u003e\n\u003ctd\u003eDNA Topoisomerases\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOrganism\u003c\/th\u003e\n\u003ctd\u003eE. coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConcentration\u003c\/th\u003e\n\u003ctd\u003e4 µM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSizes\u003c\/th\u003e\n\u003ctd\u003e100 assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog #\u003c\/th\u003e\n\u003ctd\u003eTOP1-100EC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm storage conditions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eShipped frozen.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eUse With\u003c\/h2\u003e\n\u003cp\u003eThis enzyme is used in the following ProFoldin assay kits:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca href=\"\/products\/96-well-e-coli-topo-i-dna-decatenation-assay-kit-plus-bht16300060\"\u003e96-Well E. coli Topo I DNA Decatenation Assay Kit Plus\u003c\/a\u003e (BHT16300060) — complete kit: already includes this enzyme\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"\/products\/dna-topoisomerase-i-assay-kit-bht16300061\"\u003eDNA Topoisomerase I Assay Kit\u003c\/a\u003e (BHT16300061) — DRA100KE already includes this enzyme; DRA100K is enzyme-free and needs this product\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"\/products\/gel-based-e-coli-topoisomerase-i-dna-relaxation-assay-kit-bht16300062\"\u003eGel-Based E. coli Topoisomerase I DNA Relaxation Assay Kit\u003c\/a\u003e (BHT16300062) — complete kit: already includes this enzyme\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtocol\u003c\/h2\u003e\n\u003cp\u003eNo separate datasheet is available for this enzyme. The ProFoldin protocol “E. coli DNA Topoisomerase I Assay” (linked under Documents) shows how the enzyme is used in the matching assay kit.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eFor Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. This enzyme is shipped frozen. Follow standard laboratory safety practices.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ProFoldin","offers":[{"title":"100 assays","offer_id":67815135215981,"sku":"TOP1-100EC","price":570.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/e-coli-dna-topoisomerase-i-bhp16300028","provider":"BioHippo","version":"1.0","type":"link"}