{"product_id":"e-coli-dna-topoisomerase-iv-bhp16300040","title":"E. coli DNA Topoisomerase IV","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ol,.bhp-desc ul{margin:0 0 12px 20px}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eEnzyme\u003c\/span\u003e\u003cp\u003eBacterial topoisomerase IV (the ParC-ParE complex) is a type II topoisomerase. It unlinks (decatenates) interlinked daughter DNA molecules at the end of DNA replication, and it also relaxes supercoiled DNA.\u003c\/p\u003e\n\u003cp\u003eSome inhibitors, such as fluoroquinolones, block the DNA rejoining step of type II topoisomerases; the cut DNA then stays bound to the enzyme as a covalent cleavage complex.\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctr\u003e\n\u003cth\u003e100 assays – TOP4-100EC\u003c\/th\u003e\n\u003ctd\u003e50 µl of 5 µM E. coli topoisomerase IV (ParC-ParE).\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/table\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eProduct Type\u003c\/th\u003e\n\u003ctd\u003eDNA Topoisomerases\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOrganism\u003c\/th\u003e\n\u003ctd\u003eE. coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConcentration\u003c\/th\u003e\n\u003ctd\u003e5 µM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative Names\u003c\/th\u003e\n\u003ctd\u003eTopoisomerase IV Holoenzyme, ParC-ParE Complex\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSizes\u003c\/th\u003e\n\u003ctd\u003e100 assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog #\u003c\/th\u003e\n\u003ctd\u003eTOP4-100EC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm storage conditions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eShipped frozen.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eUse With\u003c\/h2\u003e\n\u003cp\u003eThis enzyme is used in the following ProFoldin assay kits:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca href=\"\/products\/96-well-e-coli-topo-iv-dna-cleavage-assay-kit-plus-bht16300076\"\u003e96-Well E. coli Topo IV DNA Cleavage Assay Kit Plus\u003c\/a\u003e (BHT16300076) — complete kit: already includes this enzyme\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"\/products\/96-well-e-coli-topo-iv-dna-decatenation-assay-kit-plus-bht16300077\"\u003e96-Well E. coli Topo IV DNA Decatenation Assay Kit Plus\u003c\/a\u003e (BHT16300077) — complete kit: already includes this enzyme\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"\/products\/gel-based-e-coli-topoisomerase-iv-dna-decatenation-assay-kit-plus-bht16300078\"\u003eGel-Based E. coli Topoisomerase IV DNA Decatenation Assay Kit Plus\u003c\/a\u003e (BHT16300078) — complete kit: already includes this enzyme\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"\/products\/dna-topoisomerase-dna-decatenation-assay-kit-bht16300083\"\u003eDNA Topoisomerase DNA Decatenation Assay Kit\u003c\/a\u003e (BHT16300083) — DDC020KE, DDC100KE already include this enzyme; DDC020K is enzyme-free and needs this product\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"\/products\/e-coli-topo-iv-atpase-assay-kit-plus-bht16300084\"\u003eE. coli Topo IV ATPase Assay Kit Plus\u003c\/a\u003e (BHT16300084) — complete kit: already includes this enzyme\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"\/products\/e-coli-topo-iv-dna-relaxation-assay-kit-plus-bht16300085\"\u003eE. coli Topo IV DNA Relaxation Assay Kit Plus\u003c\/a\u003e (BHT16300085) — complete kit: already includes this enzyme\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtocol\u003c\/h2\u003e\n\u003cp\u003eNo separate datasheet is available for this enzyme. The ProFoldin protocols “Topoisomerase DNA Cleavage Assays”, “Gel-Based E. coli Topoisomerase IV DNA Decatenation Assay”, “DNA Topoisomerase IV Assay”, “DNA Topoisomerase ATPase Assays” and “Topo IV DNA Relaxation Assays” (linked under Documents) show how the enzyme is used in the matching assay kits.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eFor Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. This enzyme is shipped frozen. Follow standard laboratory safety practices.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ProFoldin","offers":[{"title":"100 assays","offer_id":67815135445357,"sku":"TOP4-100EC","price":570.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/e-coli-dna-topoisomerase-iv-bhp16300040","provider":"BioHippo","version":"1.0","type":"link"}