{"product_id":"e-coli-murf-assay-kit-plus-bht16300040","title":"E. coli MurF Assay Kit Plus","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ol,.bhp-desc ul{margin:0 0 12px 20px}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eDrug Target Assay Kit\u003c\/span\u003e\u003cp\u003eE. coli MurF is a bacterial cell wall synthesis enzyme and a target for antibacterial drug discovery.\u003c\/p\u003e\n\u003ch2\u003ePack Sizes\u003c\/h2\u003e\n\u003cp\u003eBoth sizes include the E. coli MurF enzyme. The only difference is the number of assays: MURF100KE is for 100 assays and MURF500KE for 500 assays (384-well format); see the component table for the volumes of each size.\u003c\/p\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003e100 assays (MURF100KE)\u003c\/th\u003e\n\u003ctd\u003e100 assays; enzyme included\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003e500 assays (MURF500KE)\u003c\/th\u003e\n\u003ctd\u003e500 assays; enzyme included\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003e100 assays (MURF100KE)\u003c\/th\u003e\n\u003cth\u003e500 assays (MURF500KE)\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10 x Buffer\u003c\/td\u003e\n\u003ctd\u003e400 µl\u003c\/td\u003e\n\u003ctd\u003e2 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e100 x UDP-MurNAc-tripeptide (UMAG-DAP)\u003c\/td\u003e\n\u003ctd\u003e35 µl\u003c\/td\u003e\n\u003ctd\u003e170 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e100 x D-Ala-D-Ala\u003c\/td\u003e\n\u003ctd\u003e35 µl\u003c\/td\u003e\n\u003ctd\u003e170 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e100 x ATP\u003c\/td\u003e\n\u003ctd\u003e35 µl\u003c\/td\u003e\n\u003ctd\u003e170 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e1000 x E. coli MurF (5000 nM)\u003c\/td\u003e\n\u003ctd\u003e10 µl\u003c\/td\u003e\n\u003ctd\u003e20 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePhosphate detection dye\u003c\/td\u003e\n\u003ctd\u003e5 ml\u003c\/td\u003e\n\u003ctd\u003e25 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Target\u003c\/th\u003e\n\u003ctd\u003eE. coli MurF activity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget Type\u003c\/th\u003e\n\u003ctd\u003eLigase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOrganism\u003c\/th\u003e\n\u003ctd\u003eE. coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Type\u003c\/th\u003e\n\u003ctd\u003eEnzyme Activity Assay; Inhibitor Screening Assay\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePlate Format\u003c\/th\u003e\n\u003ctd\u003e384-well plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSizes\u003c\/th\u003e\n\u003ctd\u003e100 assays, 500 assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm storage conditions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eShipped frozen.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eProtocol\u003c\/h2\u003e\n\u003cp\u003eA detailed protocol is not available online for this kit. Please contact us and we will request the instructions from the manufacturer for you.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eFor Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. The E. coli MurF Assay Kit Plus is shipped frozen. Follow standard laboratory safety practices when handling the reagents. Please contact us to confirm storage conditions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ProFoldin","offers":[{"title":"100 assays","offer_id":67794877776237,"sku":"MURF100KE","price":829.0,"currency_code":"USD","in_stock":true},{"title":"500 assays","offer_id":67794883346797,"sku":"MURF500KE","price":2644.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/MurF_copy_2_51d83be8-acf7-45b5-98c1-df9aa5bc2632.jpg?v=1792791969","url":"https:\/\/www.ebiohippo.com\/products\/e-coli-murf-assay-kit-plus-bht16300040","provider":"BioHippo","version":"1.0","type":"link"}