{"product_id":"easystep-human-vegfa-vascular-endothelial-growth-factor-a-elisa-kit-bhe15208356","title":"EasyStep Human VEGFA(Vascular Endothelial Growth Factor A) ELISA Kit","description":"\u003ch3\u003eScientific background\u003c\/h3\u003e\u003cp\u003e\u003cstrong\u003eEasyStep Human VEGFA (Vascular Endothelial Growth Factor A)\u003c\/strong\u003e is a growth factor–related marker involved in cellular proliferation, differentiation, or tissue remodeling processes.\u003c\/p\u003e\u003cp\u003eGrowth factor concentrations can reflect changes in tissue repair, fibrosis, angiogenesis, or tumor microenvironment signaling depending on the specific target and model.\u003c\/p\u003e\u003cp\u003eBecause many growth factors act locally and are regulated post-transcriptionally, protein quantification can add clarity beyond mRNA-only measurements.\u003c\/p\u003e\u003ch3\u003eWhy it matters\u003c\/h3\u003e\u003cul\u003e\n\u003cli\u003eQuantify \u003cstrong\u003eEasyStep Human VEGFA (Vascular Endothelial Growth Factor A)\u003c\/strong\u003e to compare biological changes across conditions, doses, or time points.\u003c\/li\u003e\n\u003cli\u003eGenerate concentration data from a standard curve to support biomarker and mechanistic studies.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch3\u003eHow the ELISA works\u003c\/h3\u003e\u003cp\u003eDesigned for \u003cstrong\u003eHuman\u003c\/strong\u003e samples, this kit uses a \u003cstrong\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VEGFA, and the Human VEGFA standard plate wells that pre-coated using protein-related techniques are provided separately. Standard\/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human VEGFA. After TMB substrate solution is added, only those wells that contain Human VEGFA and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VEGFA in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/strong\u003e. After binding and washing, signal is converted to concentration using a standard curve.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSample types\u003c\/strong\u003e: Serum, plasma.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection range\u003c\/strong\u003e: 78.2-5000 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSensitivity\/LoD\u003c\/strong\u003e: 31 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay time\u003c\/strong\u003e: 1.5h\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"ELK Biotechnology","offers":[{"title":"96 T","offer_id":52965951930733,"sku":"ELK033ES-96T","price":595.4,"currency_code":"USD","in_stock":true},{"title":"48 T","offer_id":52965951963501,"sku":"ELK033ES-48T","price":416.0,"currency_code":"USD","in_stock":true},{"title":"96 T X 5","offer_id":52965951996269,"sku":"ELK033ES-96TX5","price":2531.1,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1h1qbq4v21p4717oo1b_a1b20ce9-3485-4904-9555-d26514bb12f2.jpg?v=1771845496","url":"https:\/\/www.ebiohippo.com\/products\/easystep-human-vegfa-vascular-endothelial-growth-factor-a-elisa-kit-bhe15208356","provider":"BioHippo","version":"1.0","type":"link"}