{"product_id":"edem1-antibody-er-degradation-enhancing-alpha-mannosidase-like-protein-1-bha17135495","title":"EDEM1 Antibody \/ ER degradation-enhancing alpha-mannosidase-like protein 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eEDEM1 Antibody \/ ER degradation-enhancing alpha-mannosidase-like protein 1 is a anti-EDEM1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e EDEM1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eEDEM1 antibody detects ER degradation-enhancing alpha-mannosidase-like protein 1, an essential quality-control enzyme involved in the endoplasmic reticulum-associated degradation (ERAD) pathway. EDEM1 recognizes misfolded glycoproteins, trims mannose residues, and targets them for degradation via the ubiquitin-proteasome system. The EDEM1 antibody is widely used in cell biology, proteostasis, and molecular quality-control studies to examine protein folding, glycoprotein degradation, and ER stress responses.\u003cbr\u003e\u003cbr\u003eEDEM1 is encoded by the EDEM1 gene located on human chromosome 3p26.1. The protein is approximately 766 amino acids in length and belongs to the glycosyl hydrolase 47 family. EDEM1 localizes to the lumen of the endoplasmic reticulum, where it associates with chaperones such as calnexin and BiP. It functions by accelerating the removal of mannose residues from misfolded glycoproteins, signaling their export from the ER for proteasomal degradation.\u003cbr\u003e\u003cbr\u003eThe EDEM1 antibody detects an 85 kilodalton band in western blot assays and exhibits reticular ER staining under confocal microscopy. EDEM1 plays a pivotal role in maintaining proteostasis by preventing accumulation of misfolded proteins, thereby protecting cells from ER stress-induced apoptosis. Under stress conditions, expression of EDEM1 is upregulated through the unfolded protein response (UPR), particularly by transcription factors ATF6 and XBP1.\u003cbr\u003e\u003cbr\u003eDysregulation of EDEM1 contributes to neurodegenerative diseases, diabetes, and cancer, where chronic ER stress leads to cell dysfunction. In addition to its role in degradation, EDEM1 fine-tunes glycoprotein folding efficiency and influences secretion rates of correctly folded proteins. Because EDEM1 acts upstream of ERAD, it serves as an early determinant of glycoprotein fate within the secretory pathway.\u003cbr\u003e\u003cbr\u003eThrough its key role in ER quality control, EDEM1 ensures fidelity of protein folding and cellular homeostasis.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=EDEM1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=EDEM1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=EDEM1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/EDEM1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=EDEM1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047293444461,"sku":"FY12592","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_e6339925-c4e5-4cab-8f46-d6cbad9c1b49.jpg?v=1782237025","url":"https:\/\/www.ebiohippo.com\/products\/edem1-antibody-er-degradation-enhancing-alpha-mannosidase-like-protein-1-bha17135495","provider":"BioHippo","version":"1.0","type":"link"}