{"product_id":"egr2-antibody-early-growth-response-protein-2-bha17135626","title":"EGR2 Antibody \/ Early growth response protein 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eEGR2 Antibody \/ Early growth response protein 2 is a anti-EGR2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e EGR2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eEGR2 antibody detects Early growth response protein 2 (also known as Krox-20), a zinc finger transcription factor that governs peripheral nerve myelination, hindbrain segmentation, and immune regulation. Encoded by the EGR2 gene on chromosome 10q21.3, this transcription factor belongs to the early growth response family of immediate-early genes activated by mitogenic and stress stimuli. EGR2 contains three C2H2-type zinc finger motifs that bind GC-rich DNA sequences to regulate genes controlling differentiation and growth arrest. It is essential for Schwann cell maturation and myelin gene expression in the peripheral nervous system.\u003cbr\u003e\u003cbr\u003eDuring development, EGR2 plays a critical role in establishing segmental identity within the hindbrain by defining rhombomere boundaries and activating patterning genes. In Schwann cells, EGR2 drives transcription of myelin structural proteins including MPZ, PMP22, and MBP, ensuring proper myelin sheath formation and maintenance. Mutations in EGR2 cause hereditary neuropathies such as Charcot-Marie-Tooth disease type 1D and Dejerine-Sottas syndrome, underscoring its indispensable function in myelination. The protein also participates in T-cell anergy and macrophage activation by regulating cytokine gene expression.\u003cbr\u003e\u003cbr\u003eThe EGR2 antibody is widely used in neurobiology, developmental biology, and immunology to detect EGR2 protein expression and localization. Western blot analysis typically shows a 50 kilodalton band, while immunohistochemistry reveals strong nuclear staining in Schwann cells and developing neurons. Expression of EGR2 is inducible by growth factors, nerve injury, and mechanical stress, making it a sensitive marker for differentiation and regeneration. In the immune system, EGR2 acts as a transcriptional repressor in T cells to maintain peripheral tolerance by suppressing activation genes.\u003cbr\u003e\u003cbr\u003eBeyond its physiological roles, EGR2 has been implicated in tumor biology and inflammatory disease. Altered EGR2 signaling can disrupt differentiation programs and contribute to leukemogenesis or autoimmune disorders. In cancer models, EGR2 functions context-dependently as either a tumor suppressor or pro-survival factor, depending on cellular environment. The EGR2 antibody allows researchers to explore these regulatory networks, providing a means to monitor transcriptional changes during neural development and immune modulation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=EGR2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=EGR2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=EGR2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/EGR2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=EGR2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047297638765,"sku":"FY12723","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_db20fabf-df83-4a57-917f-1c50137ed658.jpg?v=1782237048","url":"https:\/\/www.ebiohippo.com\/products\/egr2-antibody-early-growth-response-protein-2-bha17135626","provider":"BioHippo","version":"1.0","type":"link"}